5wx9

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==Crystal Structure of AtERF96 with GCC-box==
==Crystal Structure of AtERF96 with GCC-box==
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<StructureSection load='5wx9' size='340' side='right' caption='[[5wx9]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
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<StructureSection load='5wx9' size='340' side='right'caption='[[5wx9]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5wx9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WX9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5wx9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WX9 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wx9 OCA], [http://pdbe.org/5wx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wx9 RCSB], [http://www.ebi.ac.uk/pdbsum/5wx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wx9 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wx9 OCA], [https://pdbe.org/5wx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wx9 RCSB], [https://www.ebi.ac.uk/pdbsum/5wx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wx9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ERF96_ARATH ERF96_ARATH]] Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity).
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[https://www.uniprot.org/uniprot/ERF96_ARATH ERF96_ARATH] Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal alpha-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal alpha-helix and AP2/ERF domain.
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Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box.,Chen CY, Lin PH, Chen KH, Cheng YS Plant Mol Biol. 2020 Nov;104(4-5):483-498. doi: 10.1007/s11103-020-01052-5. Epub , 2020 Aug 19. PMID:32813232<ref>PMID:32813232</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5wx9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Chen, C Y]]
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[[Category: Arabidopsis thaliana]]
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[[Category: Cheng, Y S]]
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[[Category: Large Structures]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Chen CY]]
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[[Category: Protein-dna complex]]
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[[Category: Cheng YS]]

Current revision

Crystal Structure of AtERF96 with GCC-box

PDB ID 5wx9

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