5y87

<StructureSection load='5y87' size='340' side='right' caption='[[5y87]], [[Resolution|resolution]] 2.13&Aring;' scene=''>

<table><tr><td colspan='2'>[[5y87]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y87 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y87 OCA], [http://pdbe.org/5y87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y87 RCSB], [http://www.ebi.ac.uk/pdbsum/5y87 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y87 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Here we report on the crystal structure and cleavage assays of a four-way junctional twister-sister self-cleaving ribozyme. Notably, 11 conserved spatially separated loop nucleotides are brought into close proximity at the ribozyme core through long-range interactions mediated by hydrated Mg2+ cations. The C62-A63 step at the cleavage site adopts a splayed-apart orientation, with flexible C62 directed outwards, whereas A63 is directed inwards and anchored by stacking and hydrogen-bonding interactions. Structure-guided studies of key base, sugar, and phosphate mutations in the twister-sister ribozyme, suggest contributions to the cleavage chemistry from interactions between a guanine at the active site and the non-bridging oxygen of the scissile phosphate, a feature found previously also for the related twister ribozyme. Our four-way junctional pre-catalytic structure differs significantly in the alignment at the cleavage step (splayed-apart vs. base-stacked) and surrounding residues and hydrated Mg2+ ions relative to a reported three-way junctional pre-catalytic structure of the twister-sister ribozyme.

 

== References ==

[[Category: Micura, R L]]

[[Category: Ren, A]]

[[Category: Zheng, L]]

[[Category: Catalysis]]

[[Category: Mn2+ soaking]]

[[Category: Twister-sister]]