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| | ==Crystal structure analysis of eukaryotic translation initiation factor 5B structure I== | | ==Crystal structure analysis of eukaryotic translation initiation factor 5B structure I== |
| - | <StructureSection load='3wbi' size='340' side='right' caption='[[3wbi]], [[Resolution|resolution]] 2.35Å' scene=''> | + | <StructureSection load='3wbi' size='340' side='right'caption='[[3wbi]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wbi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WBI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WBI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wbi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WBI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wbj|3wbj]], [[3wbk|3wbk]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FUN12, YAL035W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wbi OCA], [https://pdbe.org/3wbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wbi RCSB], [https://www.ebi.ac.uk/pdbsum/3wbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wbi ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wbi OCA], [http://pdbe.org/3wbi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wbi RCSB], [http://www.ebi.ac.uk/pdbsum/3wbi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wbi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IF2P_YEAST IF2P_YEAST]] Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. | + | [https://www.uniprot.org/uniprot/IF2P_YEAST IF2P_YEAST] Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 3wbi" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 3wbi" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Ose, T]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Tanaka, I]] | + | [[Category: Ose T]] |
| - | [[Category: Yamamoto, R]] | + | [[Category: Tanaka I]] |
| - | [[Category: Yao, M]] | + | [[Category: Yamamoto R]] |
| - | [[Category: Yu, J]] | + | [[Category: Yao M]] |
| - | [[Category: Zheng, A]] | + | [[Category: Yu J]] |
| - | [[Category: Biosynthetic protein]]
| + | [[Category: Zheng A]] |
| - | [[Category: Eif1a]]
| + | |
| - | [[Category: Eukaryotic translation initiation]]
| + | |
| - | [[Category: Flexible]]
| + | |
| Structural highlights
Function
IF2P_YEAST Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.
Publication Abstract from PubMed
eIF5B and eIF1A are two translation-initiation factors that are universally conserved among all kingdoms. They show a unique interaction in eukaryotes which is important for ribosomal subunit joining. Here, the structures of two isolated forms of yeast eIF5B and of the eIF5B-eIF1A complex (eIF1A and eIF5B do not contain the respective N-terminal domains) are reported. The eIF5B-eIF1A structure shows that the C-terminal tail of eIF1A binds to eIF5B domain IV, while the core domain of eIF1A is invisible in the electron-density map. Although the individual domains in all structures of eIF5B or archaeal IF5B (aIF5B) are similar, their domain arrangements are significantly different, indicating high structural flexibility, which is advantageous for conformational change during ribosomal subunit joining. Based on these structures, models of eIF5B, eIF1A and tRNAi(Met) on the 80S ribosome were built. The models suggest that the interaction between the eIF1A C-terminal tail and eIF5B helps tRNAi(Met) to bind to eIF5B domain IV, thus preventing tRNAi(Met) dissociation, stabilizing the interface for subunit joining and providing a checkpoint for correct ribosome assembly.
X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A.,Zheng A, Yu J, Yamamoto R, Ose T, Tanaka I, Yao M Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3090-8. doi:, 10.1107/S1399004714021476. Epub 2014 Nov 22. PMID:25478828[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zheng A, Yu J, Yamamoto R, Ose T, Tanaka I, Yao M. X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3090-8. doi:, 10.1107/S1399004714021476. Epub 2014 Nov 22. PMID:25478828 doi:http://dx.doi.org/10.1107/S1399004714021476
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