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| | ==X-ray Crystal Structure of PA protein from Influenza strain H7N9== | | ==X-ray Crystal Structure of PA protein from Influenza strain H7N9== |
| - | <StructureSection load='4p9a' size='340' side='right' caption='[[4p9a]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='4p9a' size='340' side='right'caption='[[4p9a]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p9a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/chicken/zhejiang/dtid-zju01/2013(h7n9)) Influenza a virus (a/chicken/zhejiang/dtid-zju01/2013(h7n9))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p9a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/chicken/Zhejiang/DTID-ZJU01/2013(H7N9)) Influenza A virus (A/chicken/Zhejiang/DTID-ZJU01/2013(H7N9))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P9A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1999Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1322048 Influenza A virus (A/chicken/Zhejiang/DTID-ZJU01/2013(H7N9))])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9a OCA], [http://pdbe.org/4p9a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p9a RCSB], [http://www.ebi.ac.uk/pdbsum/4p9a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9a ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9a OCA], [https://pdbe.org/4p9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p9a RCSB], [https://www.ebi.ac.uk/pdbsum/4p9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/M9V5W3_9INFA M9V5W3_9INFA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4p9a" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4p9a" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Clifton, M C]] | + | [[Category: Large Structures]] |
| - | [[Category: Edwards, T E]] | + | [[Category: Clifton MC]] |
| - | [[Category: Fairman, J W]] | + | [[Category: Edwards TE]] |
| - | [[Category: Lorimer, D]] | + | [[Category: Fairman JW]] |
| - | [[Category: Moen, S O]] | + | [[Category: Lorimer D]] |
| - | [[Category: Structural genomic]] | + | [[Category: Moen SO]] |
| - | [[Category: Pa protein influenza h7n9 polymerase]]
| + | |
| - | [[Category: Ssgcid]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Function
M9V5W3_9INFA
Publication Abstract from PubMed
Influenza A viruses cause the respiratory illness influenza, which can be mild to fatal depending on the strain and host immune response. The flu polymerase acidic (PA), polymerase basic 1 (PB1), and polymerase basic 2 (PB2) proteins comprise the RNA-dependent RNA polymerase complex responsible for viral genome replication. The first crystal structures of the C-terminal domain of PA (PA-CTD) in the absence of PB1-derived peptides show a number of structural changes relative to the previously reported PB1-peptide bound structures. The human A/WSN/1933 (H1N1) and avian A/Anhui1/2013 (H7N9) strain PA-CTD proteins exhibit the same global topology as other strains in the absence of PB1, but differ extensively in the PB1 binding pocket including a widening of the binding groove and the unfolding of a beta-turn. Both PA-CTD proteins exhibited a significant increase in thermal stability in the presence of either a PB1-derived peptide or a previously reported inhibitor in differential scanning fluorimetry assays. These structural changes demonstrate plasticity in the PA-PB1 binding interface which may be exploited in the development of novel therapeutics.
Structural analysis of H1N1 and H7N9 influenza A virus PA in the absence of PB1.,Moen SO, Abendroth J, Fairman JW, Baydo RO, Bullen J, Kirkwood JL, Barnes SR, Raymond AC, Begley DW, Henkel G, McCormack K, Tam VC, Phan I, Staker BL, Stacy R, Myler PJ, Lorimer D, Edwards TE Sci Rep. 2014 Aug 4;4:5944. doi: 10.1038/srep05944. PMID:25089892[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Moen SO, Abendroth J, Fairman JW, Baydo RO, Bullen J, Kirkwood JL, Barnes SR, Raymond AC, Begley DW, Henkel G, McCormack K, Tam VC, Phan I, Staker BL, Stacy R, Myler PJ, Lorimer D, Edwards TE. Structural analysis of H1N1 and H7N9 influenza A virus PA in the absence of PB1. Sci Rep. 2014 Aug 4;4:5944. doi: 10.1038/srep05944. PMID:25089892 doi:http://dx.doi.org/10.1038/srep05944
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