2bfy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:01, 6 November 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2bfy.gif|left|200px]]
 
-
{{Structure
+
==Complex of Aurora-B with INCENP and Hesperadin.==
-
|PDB= 2bfy |SIZE=350|CAPTION= <scene name='initialview01'>2bfy</scene>, resolution 1.80&Aring;
+
<StructureSection load='2bfy' size='340' side='right'caption='[[2bfy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=AC1:H1n+Binding+Site+For+Chain+B'>AC1</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=H1N:N-[2-OXO-3-((E)-PHENYL{[4-(PIPERIDIN-1-YLMETHYL)PHENYL]IMINO}METHYL)-2,6-DIHYDRO-1H-INDOL-5-YL]ETHANESULFONAMIDE'>H1N</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>
+
<table><tr><td colspan='2'>[[2bfy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFY FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H1N:N-[2-OXO-3-((E)-PHENYL{[4-(PIPERIDIN-1-YLMETHYL)PHENYL]IMINO}METHYL)-2,6-DIHYDRO-1H-INDOL-5-YL]ETHANESULFONAMIDE'>H1N</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfy OCA], [https://pdbe.org/2bfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfy RCSB], [https://www.ebi.ac.uk/pdbsum/2bfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfy ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfy OCA], [http://www.ebi.ac.uk/pdbsum/2bfy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bfy RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/AUKBA_XENLA AUKBA_XENLA] Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Phosphorylates 'Ser-10' of histone H3 during mitosis.<ref>PMID:12221116</ref> <ref>PMID:11350965</ref> <ref>PMID:17199039</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfy_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bfy ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Aurora family serine/threonine kinases control mitotic progression, and their deregulation is implicated in tumorigenesis. Aurora A and Aurora B, the best-characterized members of mammalian Aurora kinases, are approximately 60% identical but bind to unrelated activating subunits. The structure of the complex of Aurora A with the TPX2 activator has been reported previously. Here, we report the crystal structure of Aurora B in complex with the IN-box segment of the inner centromere protein (INCENP) activator and with the small molecule inhibitor Hesperadin. The Aurora B:INCENP complex is remarkably different from the Aurora A:TPX2 complex. INCENP forms a crown around the small lobe of Aurora B and induces the active conformation of the T loop allosterically. The structure represents an intermediate state of activation of Aurora B in which the Aurora B C-terminal segment stabilizes an open conformation of the catalytic cleft, and a critical ion pair in the kinase active site is impaired. Phosphorylation of two serines in the carboxyl terminus of INCENP generates the fully active kinase.
-
'''COMPLEX OF AURORA-B WITH INCENP AND HESPERIDIN.'''
+
Mechanism of Aurora B activation by INCENP and inhibition by hesperadin.,Sessa F, Mapelli M, Ciferri C, Tarricone C, Areces LB, Schneider TR, Stukenberg PT, Musacchio A Mol Cell. 2005 Apr 29;18(3):379-91. PMID:15866179<ref>PMID:15866179</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2bfy" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
Aurora family serine/threonine kinases control mitotic progression, and their deregulation is implicated in tumorigenesis. Aurora A and Aurora B, the best-characterized members of mammalian Aurora kinases, are approximately 60% identical but bind to unrelated activating subunits. The structure of the complex of Aurora A with the TPX2 activator has been reported previously. Here, we report the crystal structure of Aurora B in complex with the IN-box segment of the inner centromere protein (INCENP) activator and with the small molecule inhibitor Hesperadin. The Aurora B:INCENP complex is remarkably different from the Aurora A:TPX2 complex. INCENP forms a crown around the small lobe of Aurora B and induces the active conformation of the T loop allosterically. The structure represents an intermediate state of activation of Aurora B in which the Aurora B C-terminal segment stabilizes an open conformation of the catalytic cleft, and a critical ion pair in the kinase active site is impaired. Phosphorylation of two serines in the carboxyl terminus of INCENP generates the fully active kinase.
+
*[[Centromere protein 3D structure|Centromere protein 3D structure]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
2BFY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFY OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
[[Category: Large Structures]]
-
Mechanism of Aurora B activation by INCENP and inhibition by hesperadin., Sessa F, Mapelli M, Ciferri C, Tarricone C, Areces LB, Schneider TR, Stukenberg PT, Musacchio A, Mol Cell. 2005 Apr 29;18(3):379-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15866179 15866179]
+
-
[[Category: Protein complex]]
+
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
-
[[Category: Areces, L B.]]
+
[[Category: Areces LB]]
-
[[Category: Ciferri, C.]]
+
[[Category: Ciferri C]]
-
[[Category: Mapelli, M.]]
+
[[Category: Mapelli M]]
-
[[Category: Musacchio, A.]]
+
[[Category: Musacchio A]]
-
[[Category: Schneider, T R.]]
+
[[Category: Schneider TR]]
-
[[Category: Sessa, F.]]
+
[[Category: Sessa F]]
-
[[Category: Stukenberg, P T.]]
+
[[Category: Stukenberg PT]]
-
[[Category: Tarricone, C.]]
+
[[Category: Tarricone C]]
-
[[Category: inhibition]]
+
-
[[Category: kinase]]
+
-
[[Category: mitosis]]
+
-
[[Category: transferase complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:05:48 2008''
+

Current revision

Complex of Aurora-B with INCENP and Hesperadin.

PDB ID 2bfy

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bfy"
Personal tools