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| | ==1.7 Angstrom Crystal Structure of leukotoxin LukD from Staphylococcus aureus.== | | ==1.7 Angstrom Crystal Structure of leukotoxin LukD from Staphylococcus aureus.== |
| - | <StructureSection load='4q7g' size='340' side='right' caption='[[4q7g]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4q7g' size='340' side='right'caption='[[4q7g]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4q7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staas Staas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4q7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MSSA476 Staphylococcus aureus subsp. aureus MSSA476]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q7G FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hlgB, lukD, lukDv, SAS1748 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=282459 STAAS])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7g OCA], [http://pdbe.org/4q7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q7g RCSB], [http://www.ebi.ac.uk/pdbsum/4q7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7g ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7g OCA], [https://pdbe.org/4q7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q7g RCSB], [https://www.ebi.ac.uk/pdbsum/4q7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7g ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LUKDV_STAAS LUKDV_STAAS]] Part of a bi-component leucotoxin that acts by forming pores in the membrane of the target cells. The activity of LukEv-LukDv to rabbit leukocytes is similar to that of the Panton-Valentine leucocidin (PVL). LukEv-LukDv is hemolytic to rabbit red blood cells although the activity is only 8% of gamma-hemolysin (By similarity). | + | [https://www.uniprot.org/uniprot/A0A0H2WX25_STAAC A0A0H2WX25_STAAC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staas]] | + | [[Category: Large Structures]] |
| - | [[Category: Anderson, W F]] | + | [[Category: Staphylococcus aureus subsp. aureus MSSA476]] |
| - | [[Category: Bagnoli, F]] | + | [[Category: Anderson WF]] |
| - | [[Category: Bottomley, M]] | + | [[Category: Bagnoli F]] |
| - | [[Category: Structural genomic]] | + | [[Category: Bottomley M]] |
| - | [[Category: Falugi, F]] | + | [[Category: Falugi F]] |
| - | [[Category: Grandi, G]] | + | [[Category: Grandi G]] |
| - | [[Category: Kwon, K]] | + | [[Category: Kwon K]] |
| - | [[Category: Minasov, G]] | + | [[Category: Minasov G]] |
| - | [[Category: Nocadello, S]] | + | [[Category: Nocadello S]] |
| - | [[Category: Shatsman, S]] | + | [[Category: Shatsman S]] |
| - | [[Category: Shuvalova, L]] | + | [[Category: Shuvalova L]] |
| - | [[Category: Csgid]]
| + | |
| - | [[Category: Distorted sandwich]]
| + | |
| - | [[Category: Leukocidin]]
| + | |
| - | [[Category: Leukocidin-like]]
| + | |
| - | [[Category: National institute of allergy and infectious disease]]
| + | |
| - | [[Category: Niaid]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
A0A0H2WX25_STAAC
Publication Abstract from PubMed
Staphylococcal leukotoxins are a family of beta-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins that Staphylococcus aureus produces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays in S. aureus pathogenesis and the identification of its protein receptors, combined with its presence in S. aureus methicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. The two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.
Crystal structures of the components of the Staphylococcus aureus leukotoxin ED.,Nocadello S, Minasov G, Shuvalova L, Dubrovska I, Sabini E, Bagnoli F, Grandi G, Anderson WF Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):113-20. doi:, 10.1107/S2059798315023207. Epub 2016 Jan 1. PMID:26894539[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nocadello S, Minasov G, Shuvalova L, Dubrovska I, Sabini E, Bagnoli F, Grandi G, Anderson WF. Crystal structures of the components of the Staphylococcus aureus leukotoxin ED. Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):113-20. doi:, 10.1107/S2059798315023207. Epub 2016 Jan 1. PMID:26894539 doi:http://dx.doi.org/10.1107/S2059798315023207
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