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| | ==Crystal Structure of Phosphoglucomutase from Xanthomonas citri complexed with glucose-1-phosphate== | | ==Crystal Structure of Phosphoglucomutase from Xanthomonas citri complexed with glucose-1-phosphate== |
| - | <StructureSection load='5bmp' size='340' side='right' caption='[[5bmp]], [[Resolution|resolution]] 1.85Å' scene=''> | + | <StructureSection load='5bmp' size='340' side='right'caption='[[5bmp]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5bmp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanac Xanac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BMP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5bmp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BMP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5bmn|5bmn]], [[5bmr|5bmr]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bmp OCA], [https://pdbe.org/5bmp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bmp RCSB], [https://www.ebi.ac.uk/pdbsum/5bmp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bmp ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xanA, XAC3579 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190486 XANAC])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglucomutase Phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.2 5.4.2.2] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bmp OCA], [http://pdbe.org/5bmp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bmp RCSB], [http://www.ebi.ac.uk/pdbsum/5bmp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bmp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8PGN7_XANAC Q8PGN7_XANAC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Phosphoglucomutase]] | + | [[Category: Large Structures]] |
| - | [[Category: Xanac]] | + | [[Category: Xanthomonas citri pv. citri str. 306]] |
| - | [[Category: Goto, L S]] | + | [[Category: Goto LS]] |
| - | [[Category: Mansur, M T.M Novo]] | + | [[Category: Novo Mansur MTM]] |
| - | [[Category: Pereira, H M]] | + | [[Category: Pereira HM]] |
| - | [[Category: Isomerase]]
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| - | [[Category: Phosphoglucomutase citrus canker]]
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| Structural highlights
Function
Q8PGN7_XANAC
Publication Abstract from PubMed
Citrus canker, caused by bacteria Xanthomonas citri subsp. citri, can affect all economically important varieties of citrus. Studying Xanthomonas genes related to the invasive capacity may improve the knowledge on how this works and ultimately use the information to avoid the disease. Some annotated genes from Xanthomonas citri subsp. citri published genome are addressed to an interesting class of genes named "pathogenicity, virulence and adaptation". One of them is xanA, which encodes a predicted phosphoglucomutase. Phosphoglucomutases are ubiquitous enzymes among the living kingdoms that play roles in carbohydrate metabolism, catalyzing the reversible conversion of 1- to 6-phosphoglucose. In Xanthomonas, phosphoglucomutase activity is required to synthesize precursors of the pathogenesis-related polysaccharide xanthan. In this work, a characterization of this gene product is presented by structural and functional studies. Molecular cloning was used for heterologous expression and deletion of xanA. A Michaelis-Menten kinetics model was obtained using the recombinant protein. The protein structure was also determined by X-ray diffraction on the recombinant enzyme substrate-free, bound to glucose-1,6-biphosphate and to glucose-1-phosphate. Deletion of xanA was done with a suicide plasmid construct and the obtained mutant was tested for pathogenic capacity. This study is the first describing the properties of the Xanthomonas citri subsp. citri phosphoglucomutase.
Structural and functional characterization of the phosphoglucomutase from Xanthomonas citri subsp. citri.,Seiji Goto L, Vessoni Alexandrino A, Malvessi Pereira C, Silva Martins C, D'Muniz Pereira H, Brandao-Neto J, Marques Novo-Mansur MT Biochim Biophys Acta. 2016 Aug 25. pii: S1570-9639(16)30170-4. doi:, 10.1016/j.bbapap.2016.08.014. PMID:27567706[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Seiji Goto L, Vessoni Alexandrino A, Malvessi Pereira C, Silva Martins C, D'Muniz Pereira H, Brandao-Neto J, Marques Novo-Mansur MT. Structural and functional characterization of the phosphoglucomutase from Xanthomonas citri subsp. citri. Biochim Biophys Acta. 2016 Aug 25. pii: S1570-9639(16)30170-4. doi:, 10.1016/j.bbapap.2016.08.014. PMID:27567706 doi:http://dx.doi.org/10.1016/j.bbapap.2016.08.014
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