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| | ==Crystal structure of Mycobacterium tuberculosis Rv3249c transcriptional regulator.== | | ==Crystal structure of Mycobacterium tuberculosis Rv3249c transcriptional regulator.== |
| - | <StructureSection load='5d1w' size='340' side='right' caption='[[5d1w]], [[Resolution|resolution]] 3.59Å' scene=''> | + | <StructureSection load='5d1w' size='340' side='right'caption='[[5d1w]], [[Resolution|resolution]] 3.59Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5d1w]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D1W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D1W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5d1w]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D1W FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.59Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d1r|5d1r]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv3249c, LH57_17765 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d1w OCA], [https://pdbe.org/5d1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d1w RCSB], [https://www.ebi.ac.uk/pdbsum/5d1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d1w ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d1w OCA], [http://pdbe.org/5d1w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d1w RCSB], [http://www.ebi.ac.uk/pdbsum/5d1w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d1w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/O05892_MYCTU O05892_MYCTU] |
| - | The mycobacterial cell wall is critical to the virulence of these pathogens. Recent work shows that the mycobacterial membrane protein large (MmpL) family of transporters contributes to cell wall biosynthesis by exporting fatty acids and lipidic elements of the cell wall. The expression of the Mycobacterium tuberculosis MmpL proteins is controlled by a complex regulatory network, including the TetR-family transcriptional regulators Rv3249c and Rv1816. Here we report the crystal structures of these two regulators, revealing dimeric, two-domain molecules with architecture consistent with the TetR family of regulators. Buried extensively within the C-terminal regulatory domains of Rv3249c and Rv1816 we found fortuitous bound ligands, which were identified as palmitic acid (a fatty acid) and isopropyl laurate (a fatty acid ester), respectively. Our results suggest that fatty acids may be the natural ligands of these regulatory proteins. Using fluorescence polarization and electrophoretic mobility shift assays, we demonstrate the recognition of promoter and intragenic regions of multiple mmpL genes by these proteins. Binding of palmitic acid renders these regulators incapable of interacting with their respective operator DNAs, which will result in derepression of the corresponding mmpL genes. Taken together, these experiments provide new perspectives on the regulation of the MmpL family of transporters.
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| - | Structural Basis for the Regulation of the MmpL Transporters of Mycobacterium tuberculosis.,Delmar JA, Chou TH, Wright CC, Licon MH, Doh JK, Radhakrishnan A, Kumar N, Lei HT, Bolla JR, Rajashankar KR, Su CC, Purdy GE, Yu EW J Biol Chem. 2015 Sep 22. pii: jbc.M115.683797. PMID:26396194<ref>PMID:26396194</ref>
| + | ==See Also== |
| - | | + | *[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5d1w" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Myctu]] | + | [[Category: Large Structures]] |
| - | [[Category: Chou, T H]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Delmar, J]] | + | [[Category: Chou T-H]] |
| - | [[Category: Su, C C]] | + | [[Category: Delmar J]] |
| - | [[Category: Yu, E]] | + | [[Category: Su C-C]] |
| - | [[Category: Mycobacterium tuberculosis]]
| + | [[Category: Yu E]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcriptional regulator]]
| + | |
