1bk8

From Proteopedia

(Difference between revisions)

Current revision

DETERMINATION OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF AESCULUS HIPPOCASTANUM ANTIMICROBIAL PROTEIN 1 (AH-AMP1) BY 1H NMR, 25 STRUCTURES

Structural highlights

1bk8 is a 1 chain structure with sequence from Aesculus hippocastanum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 25 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DEF1_AESHI Possesses antimicrobial activity sensitive to inorganic cations. Binds specifically to the fungal plasma membrane. Has no inhibitory effect on insect gut alpha-amylase.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Aesculus hippocastanum antimicrobial protein 1 (Ah-AMP1) is a plant defensin isolated from horse chestnuts. The plant defensins have been divided in several subfamilies according to their amino acid sequence homology. Ah-AMP1, belonging to subfamily A2, inhibits growth of a broad range of fungi. So far, a three-dimensional structure has been determined only for members of subfamilies A3 and B2. In order to understand activity and specificity of these plant defensins, the structure of a protein belonging to subfamily A2 is needed. We report the three-dimensional solution structure of Ah-AMP1 as determined from two-dimensional 1H nuclear magnetic resonance data. The structure features all the characteristics of the "cysteine-stabilized alpha beta-motif." A comparison of the structure, the electrostatic potential surface and regions important for interaction with the fungal receptor, is made with Rs-AFP1 (plant defensin of subfamily A3). Thus, residues important for activity and specificity have been assigned.

The three-dimensional solution structure of Aesculus hippocastanum antimicrobial protein 1 determined by 1H nuclear magnetic resonance.,Fant F, Vranken WF, Borremans FA Proteins. 1999 Nov 15;37(3):388-403. PMID:10591099^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Thevissen K, Osborn RW, Acland DP, Broekaert WF. Specific binding sites for an antifungal plant defensin from Dahlia (Dahlia merckii) on fungal cells are required for antifungal activity. Mol Plant Microbe Interact. 2000 Jan;13(1):54-61. PMID:10656585 doi:http://dx.doi.org/10.1094/MPMI.2000.13.1.54
↑ Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S, Van Leuven F, Attenborough S, Rees SB, Broekaert WF. Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 1995 Jul 17;368(2):257-62. PMID:7628617
↑ Fant F, Vranken WF, Borremans FA. The three-dimensional solution structure of Aesculus hippocastanum antimicrobial protein 1 determined by 1H nuclear magnetic resonance. Proteins. 1999 Nov 15;37(3):388-403. PMID:10591099

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bk8"

Categories: Aesculus hippocastanum | Large Structures | Borremans FAM | Fant F

@@ Line 1: / Line 1: @@
 ==DETERMINATION OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF AESCULUS HIPPOCASTANUM ANTIMICROBIAL PROTEIN 1 (AH-AMP1) BY 1H NMR, 25 STRUCTURES==
-<StructureSection load='1bk8' size='340' side='right' caption='[[1bk8]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
+<StructureSection load='1bk8' size='340' side='right'caption='[[1bk8]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1bk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aesculus_hippocastanum Aesculus hippocastanum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BK8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1bk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aesculus_hippocastanum Aesculus hippocastanum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BK8 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bk8 OCA], [http://pdbe.org/1bk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1bk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bk8 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bk8 OCA], [https://pdbe.org/1bk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1bk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bk8 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DEF1_AESHI DEF1_AESHI]] Possesses antimicrobial activity sensitive to inorganic cations. Binds specifically to the fungal plasma membrane. Has no inhibitory effect on insect gut alpha-amylase.<ref>PMID:10656585</ref> <ref>PMID:7628617</ref>
+[https://www.uniprot.org/uniprot/DEF1_AESHI DEF1_AESHI] Possesses antimicrobial activity sensitive to inorganic cations. Binds specifically to the fungal plasma membrane. Has no inhibitory effect on insect gut alpha-amylase.<ref>PMID:10656585</ref> <ref>PMID:7628617</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/1bk8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/1bk8_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 27: / Line 28: @@
 </div>
 <div class="pdbe-citations 1bk8" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Plant defensin|Plant defensin]]
 == References ==
 <references/>
@@ Line 32: / Line 36: @@
 </StructureSection>
 [[Category: Aesculus hippocastanum]]
-[[Category: Borremans, F A.M]]
+[[Category: Large Structures]]
-[[Category: Fant, F]]
+[[Category: Borremans FAM]]
-[[Category: Antimicrobial]]
+[[Category: Fant F]]
-[[Category: Cysteine-stabilized alfa/ beta motif]]
-[[Category: Plant defensin]]

1bk8

From Proteopedia

Current revision

DETERMINATION OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF AESCULUS HIPPOCASTANUM ANTIMICROBIAL PROTEIN 1 (AH-AMP1) BY 1H NMR, 25 STRUCTURES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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