2bon

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[[Image:2bon.jpg|left|200px]]
 
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{{Structure
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==Structure of an Escherichia coli lipid kinase (YegS)==
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|PDB= 2bon |SIZE=350|CAPTION= <scene name='initialview01'>2bon</scene>, resolution 1.90&Aring;
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<StructureSection load='2bon' size='340' side='right'caption='[[2bon]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE= <scene name='pdbsite=MGB:Mg+Binding+Site+For+Chain+B'>MGB</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[2bon]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BON FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bon OCA], [https://pdbe.org/2bon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bon RCSB], [https://www.ebi.ac.uk/pdbsum/2bon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bon ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bon OCA], [http://www.ebi.ac.uk/pdbsum/2bon PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bon RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/YEGS_ECOLI YEGS_ECOLI] In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.[HAMAP-Rule:MF_01377]
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== Evolutionary Conservation ==
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'''STRUCTURE OF AN ESCHERICHIA COLI LIPID KINASE (YEGS)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/2bon_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bon ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The human lipid kinase family controls cell proliferation, differentiation, and tumorigenesis and includes diacylglycerol kinases, sphingosine kinases, and ceramide kinases. YegS is an Escherichia coli protein with significant sequence homology to the catalytic domain of the human lipid kinases. We have solved the crystal structure of YegS and shown that it is a lipid kinase with phosphatidylglycerol kinase activity. The crystal structure reveals a two-domain protein with significant structural similarity to a family of NAD kinases. The active site is located in the interdomain cleft formed by four conserved sequence motifs. Surprisingly, the structure reveals a novel metal binding site composed of residues conserved in most lipid kinases.
The human lipid kinase family controls cell proliferation, differentiation, and tumorigenesis and includes diacylglycerol kinases, sphingosine kinases, and ceramide kinases. YegS is an Escherichia coli protein with significant sequence homology to the catalytic domain of the human lipid kinases. We have solved the crystal structure of YegS and shown that it is a lipid kinase with phosphatidylglycerol kinase activity. The crystal structure reveals a two-domain protein with significant structural similarity to a family of NAD kinases. The active site is located in the interdomain cleft formed by four conserved sequence motifs. Surprisingly, the structure reveals a novel metal binding site composed of residues conserved in most lipid kinases.
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==About this Structure==
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Crystal structure of YegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site.,Bakali HM, Herman MD, Johnson KA, Kelly AA, Wieslander A, Hallberg BM, Nordlund P J Biol Chem. 2007 Jul 6;282(27):19644-52. Epub 2007 Mar 11. PMID:17351295<ref>PMID:17351295</ref>
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2BON is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BON OCA].
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==Reference==
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Crystal structure of YegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site., Bakali HM, Herman MD, Johnson KA, Kelly AA, Wieslander A, Hallberg BM, Nordlund P, J Biol Chem. 2007 Jul 6;282(27):19644-52. Epub 2007 Mar 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17351295 17351295]
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Bakali, H M.]]
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[[Category: Hallberg, B M.]]
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[[Category: Herman, M D.]]
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[[Category: Johnson, K A.]]
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[[Category: Nordlund, P.]]
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[[Category: lipid kinase,dag kinase,atp-binding]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:09:29 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2bon" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Bakali HM]]
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[[Category: Hallberg BM]]
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[[Category: Herman MD]]
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[[Category: Johnson KA]]
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[[Category: Nordlund P]]

Current revision

Structure of an Escherichia coli lipid kinase (YegS)

PDB ID 2bon

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