5yvm

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'''Unreleased structure'''
 
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The entry 5yvm is ON HOLD
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==Crystal Structure of the archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase ADH/D1 bound to NZQ==
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<StructureSection load='5yvm' size='340' side='right'caption='[[5yvm]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5yvm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidate_division_MSBL1_archaeon_SCGC-AAA259E19 Candidate division MSBL1 archaeon SCGC-AAA259E19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YVM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NZQ:5,6-DIHYDROXY-NADP'>NZQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yvm OCA], [https://pdbe.org/5yvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yvm RCSB], [https://www.ebi.ac.uk/pdbsum/5yvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yvm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A133UP32_9EURY A0A133UP32_9EURY]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Because only 0.01% of prokaryotic genospecies can be cultured and in situ observations are often impracticable, culture-independent methods are required to understand microbial life and harness potential applications of microbes. Here, we report a methodology for the production of proteins with desired functions based on single amplified genomes (SAGs) from unculturable species. We use this method to resurrect an alcohol dehydrogenase (ADH/D1) from an uncharacterized halo-thermophilic archaeon collected from a brine pool at the bottom of the Red Sea. Our crystal structure of 5,6-dihydroxy NADPH-bound ADH/D1 combined with biochemical analyses reveal the molecular features of its halo-thermophily, its unique habitat adaptations, and its possible reaction mechanism for atypical oxygen activation. Our strategy offers a general guide for using SAGs as a source for scientific and industrial investigations of "microbial dark matter."
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Authors: Grotzinger, S.W., Strillinger, E., Frank, A., Eppinger, J., Groll, M., Arold, S.T.
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Identification and Experimental Characterization of an Extremophilic Brine Pool Alcohol Dehydrogenase from Single Amplified Genomes.,Grotzinger SW, Karan R, Strillinger E, Bader S, Frank A, Al Rowaihi IS, Akal A, Wackerow W, Archer JA, Rueping M, Weuster-Botz D, Groll M, Eppinger J, Arold ST ACS Chem Biol. 2017 Dec 18. doi: 10.1021/acschembio.7b00792. PMID:29188989<ref>PMID:29188989</ref>
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Description: Crystal Structure of ADH/D1, an archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Frank, A]]
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<div class="pdbe-citations 5yvm" style="background-color:#fffaf0;"></div>
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[[Category: Strillinger, E]]
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[[Category: Groll, M]]
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==See Also==
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[[Category: Eppinger, J]]
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*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
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[[Category: Grotzinger, S.W]]
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== References ==
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[[Category: Arold, S.T]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Candidate division MSBL1 archaeon SCGC-AAA259E19]]
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[[Category: Large Structures]]
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[[Category: Arold ST]]
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[[Category: Eppinger J]]
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[[Category: Frank A]]
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[[Category: Groetzinger SW]]
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[[Category: Groll M]]
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[[Category: Strillinger E]]

Current revision

Crystal Structure of the archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase ADH/D1 bound to NZQ

PDB ID 5yvm

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