6f09
From Proteopedia
(Difference between revisions)
| (2 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide== | |
| + | <StructureSection load='6f09' size='340' side='right'caption='[[6f09]], [[Resolution|resolution]] 1.59Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6f09]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F09 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.594Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f09 OCA], [https://pdbe.org/6f09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f09 RCSB], [https://www.ebi.ac.uk/pdbsum/6f09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f09 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ubiquitin-specific protease 8 (USP8)/14-3-3 protein-protein interaction has recently been shown to exert a significant role in the pathogenesis of Cushing's disease (CD). USP8 is a deubiquitinase that prevents epidermal growth factor receptor (EGFR) degradation. Impairment of 14-3-3 binding leads to a higher deubiquitination of EGFR and results in a higher EGFR signaling and an increased production of adrenocorticotropic hormone. Here we report the high-resolution crystal structure of the 14-3-3 binding motif of USP8 surrounding Ser718 in complex with 14-3-3zeta and characterize the interaction with fluorescence polarization and isothermal titration calorimetry. Furthermore, we analyze the effect of USP8 mutations identified in CD on binding to 14-3-3. | ||
| - | + | Biophysical and structural insight into the USP8/14-3-3 interaction.,Centorrino F, Ballone A, Wolter M, Ottmann C FEBS Lett. 2018 Feb 23. doi: 10.1002/1873-3468.13017. PMID:29473952<ref>PMID:29473952</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6f09" style="background-color:#fffaf0;"></div> |
| - | [[Category: Ballone | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]] |
| - | [[Category: Leysen | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ballone A]] | ||
| + | [[Category: Centorrino F]] | ||
| + | [[Category: Guo S]] | ||
| + | [[Category: Leysen S]] | ||
| + | [[Category: Ottmann C]] | ||
Current revision
Binary complex of 14-3-3 zeta with ubiquitin specific protease 8 (USP8) pSer718 peptide
| |||||||||||
