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| | ==Crystal structure of Salmonella SafD adhesin== | | ==Crystal structure of Salmonella SafD adhesin== |
| - | <StructureSection load='5y9g' size='340' side='right' caption='[[5y9g]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='5y9g' size='340' side='right'caption='[[5y9g]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5y9g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_enteritidis"_gaertner_1888 "bacillus enteritidis" gaertner 1888]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y9G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5y9g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] and [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Enteritidis Salmonella enterica subsp. enterica serovar Enteritidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y9G FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IN36_14140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=149539 "Bacillus enteritidis" Gaertner 1888])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y9g OCA], [http://pdbe.org/5y9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y9g RCSB], [http://www.ebi.ac.uk/pdbsum/5y9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y9g ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y9g OCA], [https://pdbe.org/5y9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y9g RCSB], [https://www.ebi.ac.uk/pdbsum/5y9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y9g ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7CR56_SALTY Q7CR56_SALTY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus enteritidis gaertner 1888]] | + | [[Category: Large Structures]] |
| - | [[Category: Meng, G]] | + | [[Category: Salmonella enterica]] |
| - | [[Category: Wang, P R]] | + | [[Category: Salmonella enterica subsp. enterica serovar Enteritidis]] |
| - | [[Category: Zeng, L H]] | + | [[Category: Meng G]] |
| - | [[Category: Zhang, L]] | + | [[Category: Wang PR]] |
| - | [[Category: Biofilm formation]] | + | [[Category: Zeng LH]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Zhang L]] |
| - | [[Category: Host recognition]]
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| - | [[Category: Poly-adhesive activity]]
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| - | [[Category: Salmonella atypical fimbriae]]
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| - | [[Category: Self-associating oligomerization]]
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| Structural highlights
Function
Q7CR56_SALTY
Publication Abstract from PubMed
Pili are critical in host recognition, colonization and biofilm formation during bacterial infection. Here, we report the crystal structures of SafD-dsc and SafD-SafA-SafA (SafDAA-dsc) in Saf pili. Cell adherence assays show that SafD and SafA are both required for host recognition, suggesting a poly-adhesive mechanism for Saf pili. Moreover, the SafDAA-dsc structure, as well as SAXS characterization, reveals an unexpected inter-molecular oligomerization, prompting the investigation of Saf-driven self-association in biofilm formation. The bead/cell aggregation and biofilm formation assays are used to demonstrate the novel function of Saf pili. Structure-based mutants targeting the inter-molecular hydrogen bonds and complementary architecture/surfaces in SafDAA-dsc dimers significantly impaired the Saf self-association activity and biofilm formation. In summary, our results identify two novel functions of Saf pili: the poly-adhesive and self-associating activities. More importantly, Saf-Saf structures and functional characterizations help to define a pili-mediated inter-cellular oligomerizaiton mechanism for bacterial aggregation, colonization and ultimate biofilm formation.
Structural basis of host recognition and biofilm formation by Salmonella Saf pili.,Zeng L, Zhang L, Wang P, Meng G Elife. 2017 Nov 10;6. doi: 10.7554/eLife.28619. PMID:29125121[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zeng L, Zhang L, Wang P, Meng G. Structural basis of host recognition and biofilm formation by Salmonella Saf pili. Elife. 2017 Nov 10;6. doi: 10.7554/eLife.28619. PMID:29125121 doi:http://dx.doi.org/10.7554/eLife.28619
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