|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Hamster EprS second repeated element. NMR, 5 structures== | | ==Hamster EprS second repeated element. NMR, 5 structures== |
| - | <StructureSection load='1d2d' size='340' side='right' caption='[[1d2d]], [[NMR_Ensembles_of_Models | 5 NMR models]]' scene=''> | + | <StructureSection load='1d2d' size='340' side='right'caption='[[1d2d]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1d2d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D2D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1d2d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D2D FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1r1b|1r1b]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d2d OCA], [http://pdbe.org/1d2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1d2d RCSB], [http://www.ebi.ac.uk/pdbsum/1d2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1d2d ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d2d OCA], [https://pdbe.org/1d2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d2d RCSB], [https://www.ebi.ac.uk/pdbsum/1d2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d2d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SYEP_CRIGR SYEP_CRIGR] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 30: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cho cell lines]] | + | [[Category: Cricetulus griseus]] |
| - | [[Category: Berthonneau, E]] | + | [[Category: Large Structures]] |
| - | [[Category: Birlirakis, N]] | + | [[Category: Berthonneau E]] |
| - | [[Category: Cahuzac, B]] | + | [[Category: Birlirakis N]] |
| - | [[Category: Guittet, E]] | + | [[Category: Cahuzac B]] |
| - | [[Category: Mirande, M]] | + | [[Category: Guittet E]] |
| - | [[Category: Ligase]]
| + | [[Category: Mirande M]] |
| - | [[Category: Protein transcription]]
| + | |
| Structural highlights
Function
SYEP_CRIGR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Aminoacyl-tRNA synthetases of higher eukaryotes possess polypeptide extensions in contrast to their prokaryotic counterparts. These extra domains of poorly understood function are believed to be involved in protein-protein or protein-RNA interactions. Here we showed by gel retardation and filter binding experiments that the repeated units that build the linker region of the bifunctional glutamyl-prolyl-tRNA synthetase had a general RNA-binding capacity. The solution structure of one of these repeated motifs was also solved by NMR spectroscopy. One repeat is built around an antiparallel coiled-coil. Strikingly, the conserved lysine and arginine residues form a basic patch on one side of the structure, presenting a suitable docking surface for nucleic acids. Therefore, this repeated motif may represent a novel type of general RNA-binding domain appended to eukaryotic aminoacyl-tRNA synthetases to serve as a cis-acting tRNA-binding cofactor.
A recurrent RNA-binding domain is appended to eukaryotic aminoacyl-tRNA synthetases.,Cahuzac B, Berthonneau E, Birlirakis N, Guittet E, Mirande M EMBO J. 2000 Feb 1;19(3):445-52. PMID:10654942[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cahuzac B, Berthonneau E, Birlirakis N, Guittet E, Mirande M. A recurrent RNA-binding domain is appended to eukaryotic aminoacyl-tRNA synthetases. EMBO J. 2000 Feb 1;19(3):445-52. PMID:10654942 doi:10.1093/emboj/19.3.445
|
