6brd
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==Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complexed with rifampin and FAD== | ==Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complexed with rifampin and FAD== | ||
| - | <StructureSection load='6brd' size='340' side='right' caption='[[6brd]], [[Resolution|resolution]] 3.32Å' scene=''> | + | <StructureSection load='6brd' size='340' side='right'caption='[[6brd]], [[Resolution|resolution]] 3.32Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6brd]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5vqc 5vqc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BRD OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6brd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_venezuelae_ATCC_10712 Streptomyces venezuelae ATCC 10712]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5vqc 5vqc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BRD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=RFP:RIFAMPICIN'>RFP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.32Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=RFP:RIFAMPICIN'>RFP</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6brd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6brd OCA], [https://pdbe.org/6brd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6brd RCSB], [https://www.ebi.ac.uk/pdbsum/6brd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6brd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ROX_STRVP ROX_STRVP] Monooxygenase that can modify rifampicin, thereby inactivating its antibiotic activity (PubMed:29398560). Inactivates a broad range of rifamycin antibiotics (PubMed:29398560).<ref>PMID:29398560</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rifamycin monooxygenases (Rox) are present in a variety of environmental bacteria and are associated with decomposition of the clinically utilized antibiotic rifampin. Here we report the structure and function of a drug-inducible rox gene from Streptomyces venezuelae, which encodes a class A flavoprotein monooxygenase that inactivates a broad range of rifamycin antibiotics. Our findings describe a mechanism of rifamycin inactivation initiated by monooxygenation of the 2-position of the naphthyl group, which subsequently results in ring opening and linearization of the antibiotic. The result is an antibiotic that no longer adopts the basket-like structure essential for binding to the RNA exit tunnel of the target RpoB, thereby providing the molecular logic of resistance. This unique mechanism of enzymatic inactivation underpins the broad spectrum of rifamycin resistance mediated by Rox enzymes and presents a new antibiotic resistance mechanism not yet seen in microbial antibiotic detoxification. | ||
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| + | Rox, a Rifamycin Resistance Enzyme with an Unprecedented Mechanism of Action.,Koteva K, Cox G, Kelso JK, Surette MD, Zubyk HL, Ejim L, Stogios P, Savchenko A, Sorensen D, Wright GD Cell Chem Biol. 2018 Jan 26. pii: S2451-9456(18)30031-X. doi:, 10.1016/j.chembiol.2018.01.009. PMID:29398560<ref>PMID:29398560</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6brd" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Monooxygenase 3D structures|Monooxygenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Streptomyces venezuelae ATCC 10712]] |
| - | [[Category: Cox | + | [[Category: Anderson WF]] |
| - | [[Category: Kelso | + | [[Category: Cox G]] |
| - | [[Category: Savchenko | + | [[Category: Kelso J]] |
| - | [[Category: Stogios | + | [[Category: Savchenko A]] |
| - | [[Category: Wright | + | [[Category: Stogios PJ]] |
| - | + | [[Category: Wright GD]] | |
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Current revision
Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complexed with rifampin and FAD
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