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| | ==2.65 Angstrom resolution crystal structure of dTDP-4-dehydrorhamnose reductase (rfbD) from Bacillus anthracis str. Ames in complex with NADP== | | ==2.65 Angstrom resolution crystal structure of dTDP-4-dehydrorhamnose reductase (rfbD) from Bacillus anthracis str. Ames in complex with NADP== |
| - | <StructureSection load='3sc6' size='340' side='right' caption='[[3sc6]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='3sc6' size='340' side='right'caption='[[3sc6]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3sc6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_(strain_ames) Bacillus anthracis (strain ames)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SC6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3sc6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SC6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BAS1138, BA_1231, GBAA_1231, rfbD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198094 Bacillus anthracis (strain Ames)])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dTDP-4-dehydrorhamnose_reductase dTDP-4-dehydrorhamnose reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.133 1.1.1.133] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sc6 OCA], [https://pdbe.org/3sc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sc6 RCSB], [https://www.ebi.ac.uk/pdbsum/3sc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sc6 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sc6 OCA], [http://pdbe.org/3sc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sc6 RCSB], [http://www.ebi.ac.uk/pdbsum/3sc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sc6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A6L7H7R4_BACAN A0A6L7H7R4_BACAN] Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.[RuleBase:RU364082] |
| - | Bacillus anthracis is the causative agent of the deadly disease Anthrax. Its use in bioterrorism and its ability to re-emerge have brought renewed interest in this organism. B. anthracis is a Gram-positive bacterium that adds L-rhamnose to its cell-wall polysaccharides using the activated donor dTDP-beta-L-rhamnose. The enzymes involved in the biosynthesis of the activated donor are absent in humans, which make them ideal targets for therapeutic development to combat pathogens. Here, the 2.65 A resolution crystal structure of the fourth enzyme in the dTDP-beta-L-rhamnose-biosynthetic pathway from B. anthracis, dTDP-4-dehydro-beta-L-rhamnose reductase (RfbD), is presented in complex with NADP(+). This enzyme catalyzes the reduction of dTDP-4-dehydro-beta-L-rhamnose to dTDP-beta-L-rhamnose. Although the protein was co-crystallized in the presence of Mg(2+), the protein lacks the conserved residues that coordinate Mg(2+).
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| - | Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose reductase (RfbD).,Law A, Stergioulis A, Halavaty AS, Minasov G, Anderson WF, Kuhn ML Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):644-650. doi:, 10.1107/S2053230X17015746. Epub 2017 Nov 6. PMID:29199984<ref>PMID:29199984</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3sc6" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: DTDP-4-dehydrorhamnose reductase]] | + | [[Category: Bacillus anthracis str. Ames]] |
| - | [[Category: Anderson, W F]] | + | [[Category: Large Structures]] |
| - | [[Category: Structural genomic]] | + | [[Category: Anderson WF]] |
| - | [[Category: Halavaty, A S]] | + | [[Category: Halavaty AS]] |
| - | [[Category: Kuhn, M]] | + | [[Category: Kuhn M]] |
| - | [[Category: Minasov, G]] | + | [[Category: Minasov G]] |
| - | [[Category: Peterson, S]] | + | [[Category: Peterson S]] |
| - | [[Category: Shuvalova, L]] | + | [[Category: Shuvalova L]] |
| - | [[Category: Bacillus anthracis str. ame]]
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| - | [[Category: Catalyzes formation of dtdp-4-dehydro-6-deoxy-l-mannose]]
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| - | [[Category: Csgid]]
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| - | [[Category: Dtdp-4-dehydrorhamnose reductase]]
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| - | [[Category: Infectious disease]]
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| - | [[Category: Nadph and h+ from dtdp-6-deoxy-l-mannose and nadp+]]
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| - | [[Category: Oxidoreductase]]
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| - | [[Category: Rfbd]]
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| - | [[Category: Rhamnose biosynthetic pathway]]
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| - | [[Category: Rossmann fold]]
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