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| | ==Structure of a eukaryotic cyclic nucleotide-gated channel== | | ==Structure of a eukaryotic cyclic nucleotide-gated channel== |
| - | <StructureSection load='5h3o' size='340' side='right' caption='[[5h3o]], [[Resolution|resolution]] 3.50Å' scene=''> | + | <SX load='5h3o' size='340' side='right' viewer='molstar' caption='[[5h3o]], [[Resolution|resolution]] 3.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5h3o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H3O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5h3o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H3O FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tax-4, ZC84.2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3o OCA], [http://pdbe.org/5h3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h3o RCSB], [http://www.ebi.ac.uk/pdbsum/5h3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3o ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3o OCA], [https://pdbe.org/5h3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h3o RCSB], [https://www.ebi.ac.uk/pdbsum/5h3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CNG_CAEEL CNG_CAEEL]] Required for normal thermosensation and chemosensation sensory behavior (PubMed:8893027). Required, downstream of receptor-type guanylate cyclase gcy-9, for CO2-mediated responses in BAG neurons (PubMed:21173231). Required, downstream of receptor-type guanylate cyclase gcy-14, for alkaline pH-mediated responses in ASE-left (ASEL) neurons (PubMed:23664973). Involved in the development of ASJ sensory neuron axon during late larval stages and in the maintenance of normal axon morphology in the adult. Regulates dauer formation (PubMed:9486798). In ASI and ASJ sensory neurons, controls behavioral response to P.aeruginosa by up-regulating the transcription of daf-7, a member of the TGF-beta family (PubMed:25303524). In AWB and AWC sensory neurons, mediates the recognition of food oders which subsequently allows for the detection of preferred food sources (PubMed:25009271).<ref>PMID:21173231</ref> <ref>PMID:23664973</ref> <ref>PMID:25009271</ref> <ref>PMID:25303524</ref> <ref>PMID:8893027</ref> <ref>PMID:9486798</ref> | + | [https://www.uniprot.org/uniprot/CNG_CAEEL CNG_CAEEL] Required for normal thermosensation and chemosensation sensory behavior (PubMed:8893027). Required, downstream of receptor-type guanylate cyclase gcy-9, for CO2-mediated responses in BAG neurons (PubMed:21173231). Required, downstream of receptor-type guanylate cyclase gcy-14, for alkaline pH-mediated responses in ASE-left (ASEL) neurons (PubMed:23664973). Involved in the development of ASJ sensory neuron axon during late larval stages and in the maintenance of normal axon morphology in the adult. Regulates dauer formation (PubMed:9486798). In ASI and ASJ sensory neurons, controls behavioral response to P.aeruginosa by up-regulating the transcription of daf-7, a member of the TGF-beta family (PubMed:25303524). In AWB and AWC sensory neurons, mediates the recognition of food oders which subsequently allows for the detection of preferred food sources (PubMed:25009271).<ref>PMID:21173231</ref> <ref>PMID:23664973</ref> <ref>PMID:25009271</ref> <ref>PMID:25303524</ref> <ref>PMID:8893027</ref> <ref>PMID:9486798</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5h3o" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5h3o" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Caeel]] | + | [[Category: Caenorhabditis elegans]] |
| - | [[Category: Gong, Y]] | + | [[Category: Large Structures]] |
| - | [[Category: Li, H]] | + | [[Category: Gong Y]] |
| - | [[Category: Li, M]] | + | [[Category: Li H]] |
| - | [[Category: Li, X]] | + | [[Category: Li M]] |
| - | [[Category: Michailidis, I]] | + | [[Category: Li X]] |
| - | [[Category: Su, D]] | + | [[Category: Michailidis I]] |
| - | [[Category: Wang, S]] | + | [[Category: Su D]] |
| - | [[Category: Yang, J]] | + | [[Category: Wang S]] |
| - | [[Category: Zhou, X]] | + | [[Category: Yang J]] |
| - | [[Category: Channel]]
| + | [[Category: Zhou X]] |
| - | [[Category: Cng]]
| + | |
| - | [[Category: Open state]]
| + | |
| - | [[Category: Tax-4]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
CNG_CAEEL Required for normal thermosensation and chemosensation sensory behavior (PubMed:8893027). Required, downstream of receptor-type guanylate cyclase gcy-9, for CO2-mediated responses in BAG neurons (PubMed:21173231). Required, downstream of receptor-type guanylate cyclase gcy-14, for alkaline pH-mediated responses in ASE-left (ASEL) neurons (PubMed:23664973). Involved in the development of ASJ sensory neuron axon during late larval stages and in the maintenance of normal axon morphology in the adult. Regulates dauer formation (PubMed:9486798). In ASI and ASJ sensory neurons, controls behavioral response to P.aeruginosa by up-regulating the transcription of daf-7, a member of the TGF-beta family (PubMed:25303524). In AWB and AWC sensory neurons, mediates the recognition of food oders which subsequently allows for the detection of preferred food sources (PubMed:25009271).[1] [2] [3] [4] [5] [6]
Publication Abstract from PubMed
Cyclic-nucleotide-gated channels are essential for vision and olfaction. They belong to the voltage-gated ion channel superfamily but their activities are controlled by intracellular cyclic nucleotides instead of transmembrane voltage. Here we report a 3.5-A-resolution single-particle electron cryo-microscopy structure of a cyclic-nucleotide-gated channel from Caenorhabditis elegans in the cyclic guanosine monophosphate (cGMP)-bound open state. The channel has an unusual voltage-sensor-like domain, accounting for its deficient voltage dependence. A carboxy-terminal linker connecting S6 and the cyclic-nucleotide-binding domain interacts directly with both the voltage-sensor-like domain and the pore domain, forming a gating ring that couples conformational changes triggered by cyclic nucleotide binding to the gate. The selectivity filter is lined by the carboxylate side chains of a functionally important glutamate and three rings of backbone carbonyls. This structure provides a new framework for understanding mechanisms of ion permeation, gating and channelopathy of cyclic-nucleotide-gated channels and cyclic nucleotide modulation of related channels.
Structure of a eukaryotic cyclic-nucleotide-gated channel.,Li M, Zhou X, Wang S, Michailidis I, Gong Y, Su D, Li H, Li X, Yang J Nature. 2017 Feb 2;542(7639):60-65. doi: 10.1038/nature20819. Epub 2017 Jan 18. PMID:28099415[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hallem EA, Spencer WC, McWhirter RD, Zeller G, Henz SR, Ratsch G, Miller DM 3rd, Horvitz HR, Sternberg PW, Ringstad N. Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):254-9. doi: 10.1073/pnas.1017354108. , Epub 2010 Dec 20. PMID:21173231 doi:http://dx.doi.org/10.1073/pnas.1017354108
- ↑ Murayama T, Takayama J, Fujiwara M, Maruyama IN. Environmental alkalinity sensing mediated by the transmembrane guanylyl cyclase GCY-14 in C. elegans. Curr Biol. 2013 Jun 3;23(11):1007-12. doi: 10.1016/j.cub.2013.04.052. Epub 2013, May 9. PMID:23664973 doi:http://dx.doi.org/10.1016/j.cub.2013.04.052
- ↑ Harris G, Shen Y, Ha H, Donato A, Wallis S, Zhang X, Zhang Y. Dissecting the signaling mechanisms underlying recognition and preference of food odors. J Neurosci. 2014 Jul 9;34(28):9389-403. doi: 10.1523/JNEUROSCI.0012-14.2014. PMID:25009271 doi:http://dx.doi.org/10.1523/JNEUROSCI.0012-14.2014
- ↑ Meisel JD, Panda O, Mahanti P, Schroeder FC, Kim DH. Chemosensation of bacterial secondary metabolites modulates neuroendocrine signaling and behavior of C. elegans. Cell. 2014 Oct 9;159(2):267-80. doi: 10.1016/j.cell.2014.09.011. PMID:25303524 doi:http://dx.doi.org/10.1016/j.cell.2014.09.011
- ↑ Komatsu H, Mori I, Rhee JS, Akaike N, Ohshima Y. Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C. elegans. Neuron. 1996 Oct;17(4):707-18. PMID:8893027
- ↑ Coburn CM, Mori I, Ohshima Y, Bargmann CI. A cyclic nucleotide-gated channel inhibits sensory axon outgrowth in larval and adult Caenorhabditis elegans: a distinct pathway for maintenance of sensory axon structure. Development. 1998 Jan;125(2):249-58. PMID:9486798
- ↑ Li M, Zhou X, Wang S, Michailidis I, Gong Y, Su D, Li H, Li X, Yang J. Structure of a eukaryotic cyclic-nucleotide-gated channel. Nature. 2017 Feb 2;542(7639):60-65. doi: 10.1038/nature20819. Epub 2017 Jan 18. PMID:28099415 doi:http://dx.doi.org/10.1038/nature20819
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