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| | ==Structure of KANK1/KIF21A complex== | | ==Structure of KANK1/KIF21A complex== |
| - | <StructureSection load='5ybe' size='340' side='right' caption='[[5ybe]], [[Resolution|resolution]] 2.11Å' scene=''> | + | <StructureSection load='5ybe' size='340' side='right'caption='[[5ybe]], [[Resolution|resolution]] 2.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ybe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YBE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ybe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YBE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.111Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kank1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ybe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ybe OCA], [http://pdbe.org/5ybe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ybe RCSB], [http://www.ebi.ac.uk/pdbsum/5ybe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ybe ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ybe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ybe OCA], [https://pdbe.org/5ybe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ybe RCSB], [https://www.ebi.ac.uk/pdbsum/5ybe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ybe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6AXG6_MOUSE Q6AXG6_MOUSE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Shang, Y]] | + | [[Category: Mus musculus]] |
| - | [[Category: Weng, Z F]] | + | [[Category: Shang Y]] |
| - | [[Category: Yao, D Q]] | + | [[Category: Weng ZF]] |
| - | [[Category: Zhang, R G]] | + | [[Category: Yao DQ]] |
| - | [[Category: Zhu, J W]] | + | [[Category: Zhang RG]] |
| - | [[Category: Ankyrin repeat]] | + | [[Category: Zhu JW]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Kank1-kif21a complex]]
| + | |
| - | [[Category: Microtubule-cell adhesion crosstalk]]
| + | |
| - | [[Category: Scaffold protein]]
| + | |
| - | [[Category: Structural biology]]
| + | |
| Structural highlights
Function
Q6AXG6_MOUSE
Publication Abstract from PubMed
The crosstalk between dynamic microtubules (MTs) and the cell cortex plays important roles in cell division, polarity, and migration. A critical adaptor that links the plus ends of MTs with the cell cortex is the KN motif and ankyrin repeat domains 1 (KANK1)/kinesin family member 21A (KIF21A) complex. Genetic defects in these two proteins are associated with various cancers and developmental diseases such as congenital fibrosis of the extraocular muscles type 1 (CFEOM1). However, the molecular mechanism governing the KANK1/KIF21A interaction and the role of the conserved ankyrin (ANK) repeats in this interaction are still unclear. In this study, we present the crystal structure of the KANK1/KIF21A complex at 2.1-angstrom resolution. The structure, together with biochemical studies, revealed that a 5-helix bundle-capping domain immediately preceding the ANK repeats of KANK1 forms a structural and functional supramodule with its ANK repeats in binding to an evolutionarily conserved peptide located in the middle of KIF21A. We also show that several missense mutations present in cancer patients are located at the interface of the KANK1/KIF21A complex and destabilize its formation. In conclusion, our study elucidates the molecular basis underlying the KANK1/KIF21A interaction and also provides possible mechanistic explanations for the diseases caused by mutations in KANK1 and KIF21A.
Structural analyses of key features in the KANK1/KIF21A complex yield mechanistic insights into the crosstalk between microtubules and the cell cortex.,Weng Z, Shang Y, Yao D, Zhu J, Zhang R J Biol Chem. 2017 Nov 20. pii: M117.816017. doi: 10.1074/jbc.M117.816017. PMID:29158259[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Weng Z, Shang Y, Yao D, Zhu J, Zhang R. Structural analyses of key features in the KANK1/KIF21A complex yield mechanistic insights into the crosstalk between microtubules and the cell cortex. J Biol Chem. 2017 Nov 20. pii: M117.816017. doi: 10.1074/jbc.M117.816017. PMID:29158259 doi:http://dx.doi.org/10.1074/jbc.M117.816017
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