6b3x

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==Crystal structure of CstF-50 in complex with CstF-77==
==Crystal structure of CstF-50 in complex with CstF-77==
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<StructureSection load='6b3x' size='340' side='right' caption='[[6b3x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='6b3x' size='340' side='right'caption='[[6b3x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6b3x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B3X FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6b3x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B3X FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CSTF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b3x OCA], [http://pdbe.org/6b3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b3x RCSB], [http://www.ebi.ac.uk/pdbsum/6b3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b3x ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b3x OCA], [https://pdbe.org/6b3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b3x RCSB], [https://www.ebi.ac.uk/pdbsum/6b3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b3x ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CSTF1_HUMAN CSTF1_HUMAN]] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA. [[http://www.uniprot.org/uniprot/CSTF3_HUMAN CSTF3_HUMAN]] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.
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[https://www.uniprot.org/uniprot/CSTF1_HUMAN CSTF1_HUMAN] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cleavage stimulation factor (CstF) is a highly conserved protein complex composed of three subunits that recognizes G/U-rich sequences downstream of the polyadenylation signal of eukaryotic mRNAs. While CstF has been identified over 25 years ago, the architecture and contribution of each subunit to RNA recognition have not been fully understood. In this study, we provide a structural basis for the recruitment of CstF-50 to CstF via interaction with CstF-77 and establish that the hexameric assembly of CstF creates a high affinity platform to target various G/U-rich sequences. We further demonstrate that CstF-77 boosts the affinity of the CstF-64 RRM to the RNA targets and CstF-50 fine tunes the ability of the complex to recognize G/U sequences of certain lengths and content.
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Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals.,Yang W, Hsu PL, Yang F, Song JE, Varani G Nucleic Acids Res. 2017 Nov 24. pii: 4647674. doi: 10.1093/nar/gkx1177. PMID:29186539<ref>PMID:29186539</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6b3x" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
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[[Category: Hsu, P]]
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[[Category: Large Structures]]
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[[Category: Song, J E]]
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[[Category: Hsu P]]
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[[Category: Varani, G]]
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[[Category: Song JE]]
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[[Category: Yang, F]]
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[[Category: Varani G]]
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[[Category: Yang, W]]
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[[Category: Yang F]]
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[[Category: Gene regulation]]
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[[Category: Yang W]]
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[[Category: Scaffold protein]]
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[[Category: Wd40 fold]]
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Current revision

Crystal structure of CstF-50 in complex with CstF-77

PDB ID 6b3x

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