5maw
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==Crystal structure of the flagellin-FliS complex from Bacillus subtilis== | ==Crystal structure of the flagellin-FliS complex from Bacillus subtilis== | ||
| - | <StructureSection load='5maw' size='340' side='right' caption='[[5maw]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='5maw' size='340' side='right'caption='[[5maw]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5maw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MAW OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5maw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MAW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5maw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5maw OCA], [https://pdbe.org/5maw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5maw RCSB], [https://www.ebi.ac.uk/pdbsum/5maw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5maw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/FLA_BACSU FLA_BACSU] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes the major building block of the flagellar filament. The proteins FliW and FliS serve in the post-transcriptional control of flagellin and guide the protein to the flagellar type III secretion system (fT3SS), respectively. Here, we present the high-resolution structure of FliS/flagellin heterodimer and show that FliS and FliW bind to opposing interfaces located at the N- and C-termini of flagellin. The FliS/flagellin/FliW heterotrimer is able to interact with FlhA-C suggesting that FliW and FliS are released during flagellin export. After release, FliW and FliS are recycled to execute a new round of post-transcriptional regulation and targeting. Taken together, our study provides a mechanism explaining how FliW and FliS synchronize the production of flagellin with the capacity of the fT3SS to secrete flagellin. | ||
| + | |||
| + | FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis.,Altegoer F, Mukherjee S, Steinchen W, Bedrunka P, Linne U, Kearns DB, Bange G Sci Rep. 2018 Aug 1;8(1):11552. doi: 10.1038/s41598-018-29884-8. PMID:30068950<ref>PMID:30068950</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5maw" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Flagellar protein 3D structures|Flagellar protein 3D structures]] | ||
| + | *[[Flagellin 3D structures|Flagellin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Bacillus subtilis]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Altegoer F]] |
| - | [[Category: | + | [[Category: Bange G]] |
| - | + | ||
Current revision
Crystal structure of the flagellin-FliS complex from Bacillus subtilis
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