2ci2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:18, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ci2.jpg|left|200px]]
 
-
{{Structure
+
==CRYSTAL AND MOLECULAR STRUCTURE OF THE SERINE PROTEINASE INHIBITOR CI-2 FROM BARLEY SEEDS==
-
|PDB= 2ci2 |SIZE=350|CAPTION= <scene name='initialview01'>2ci2</scene>, resolution 2.0&Aring;
+
<StructureSection load='2ci2' size='340' side='right'caption='[[2ci2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[2ci2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ci2 1ci2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CI2 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ci2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ci2 OCA], [https://pdbe.org/2ci2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ci2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ci2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ci2 ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ci2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ci2 OCA], [http://www.ebi.ac.uk/pdbsum/2ci2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ci2 RCSB]</span>
+
[https://www.uniprot.org/uniprot/ICI2_HORVU ICI2_HORVU] Inhibits both subtilisin and chymotrypsin.
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/2ci2_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ci2 ConSurf].
 +
<div style="clear:both"></div>
-
'''CRYSTAL AND MOLECULAR STRUCTURE OF THE SERINE PROTEINASE INHIBITOR CI-2 FROM BARLEY SEEDS'''
+
==See Also==
-
 
+
*[[Chymotrypsin inhibitor 3D structures|Chymotrypsin inhibitor 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Chymotrypsin inhibitor 2 (CI-2), a serine proteinase inhibitor from barley seeds, has been crystallized and its three-dimensional structure determined at 2.0-A resolution by the molecular replacement method. The structure has been refined by restrained-parameter least-squares methods to a crystallographic R factor (= sigma parallel Fo magnitude of-Fo parallel/sigma magnitude of Fo) o of 0.198. CI-2 is a member of the potato inhibitor 1 family. It lacks the characteristic stabilizing disulfide bonds of most other members of serine proteinase inhibitor families. The body of CI-2 shows few conformational changes between the free inhibitor and the previously reported structure of CI-2 in complex with subtilisin Novo [McPhalen, C.A., Svendsen, I., Jonassen, I., &amp; James, M.N.G. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 7242-7246]. However, the reactive site loop has some significant conformational differences between the free inhibitor and its complexed form. The residues in this segment of polypeptide exhibit relatively large thermal motion parameters and some disorder in the uncomplexed form of the inhibitor. The reactive site bond is between Met-59I and Glu-60I in the consecutive sequential numbering of CI-2 (Met-60-Glu-61 according to the alignment of Svendsen et al. [Svendsen, I., Hejgaard, J., &amp; Chavan, J.K. (1984) Carlsberg Res. Commun. 49, 493-502]). The network of hydrogen bonds and electrostatic interactions stabilizing the conformation of the reactive site loop is much less extensive in the free than in the complexed inhibitor.
+
-
 
+
-
==About this Structure==
+
-
2CI2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. This structure supersedes the now removed PDB entry 1CI2. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CI2 OCA].
+
-
 
+
-
==Reference==
+
-
Crystal and molecular structure of the serine proteinase inhibitor CI-2 from barley seeds., McPhalen CA, James MN, Biochemistry. 1987 Jan 13;26(1):261-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3828302 3828302]
+
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: James, M N.G.]]
+
[[Category: James MNG]]
-
[[Category: Mcphalen, C A.]]
+
[[Category: Mcphalen CA]]
-
[[Category: proteinase inhibitor (chymotrypsin)]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:21:46 2008''
+

Current revision

CRYSTAL AND MOLECULAR STRUCTURE OF THE SERINE PROTEINASE INHIBITOR CI-2 FROM BARLEY SEEDS

PDB ID 2ci2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ci2"
Personal tools