5mn3
From Proteopedia
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==NMR structure of the Littorina littorea metallothionein, a snail MT folding into three distinct domains== | ==NMR structure of the Littorina littorea metallothionein, a snail MT folding into three distinct domains== | ||
| - | <StructureSection load='5mn3' size='340' side='right' caption='[[5mn3 | + | <StructureSection load='5mn3' size='340' side='right'caption='[[5mn3]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5mn3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MN3 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5mn3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Littorina_littorea Littorina littorea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MN3 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mn3 OCA], [https://pdbe.org/5mn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mn3 RCSB], [https://www.ebi.ac.uk/pdbsum/5mn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mn3 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In this study, we present an NMR structure of the metallothionein (MT) from the snail Littorina littorea (LlMT) in complex with Cd2+ . LlMT is capable of binding 9 Zn2+ or 9 Cd2+ ions. Sequence alignments with other snail MTs revealed that the protein is likely composed of three domains. The study revealed that the protein is divided into three individual domains, each of which folds into a single well-defined three-metal cluster. The central alpha2 and C-terminal beta domains are positioned with a unique relative orientation. Two variants with longer and shorter linkers were investigated, which revealed that specific interdomain contacts only occurred with the wild-type linker. Moreover, a domain-swap mutant in which the highly similar alpha1 and alpha2 domains were exchanged was structurally almost identical. It is suggested that the expression of a three-domain MT confers an evolutionary advantage on Littorina littorea in terms of coping with Cd2+ stress and adverse environmental conditions. | ||
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| + | Structural Adaptation of a Protein to Increased Metal Stress: NMR Structure of a Marine Snail Metallothionein with an Additional Domain.,Baumann C, Beil A, Jurt S, Niederwanger M, Palacios O, Capdevila M, Atrian S, Dallinger R, Zerbe O Angew Chem Int Ed Engl. 2017 Apr 10;56(16):4617-4622. doi:, 10.1002/anie.201611873. Epub 2017 Mar 23. PMID:28332759<ref>PMID:28332759</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5mn3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Littorina littorea]] |
| - | [[Category: | + | [[Category: Baumann C]] |
| - | [[Category: | + | [[Category: Jurt S]] |
| - | [[Category: | + | [[Category: Zerbe O]] |
Current revision
NMR structure of the Littorina littorea metallothionein, a snail MT folding into three distinct domains
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