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5xfy

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==Crystal structure of a novel PET hydrolase S131A mutant from Ideonella sakaiensis 201-F6==
==Crystal structure of a novel PET hydrolase S131A mutant from Ideonella sakaiensis 201-F6==
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<StructureSection load='5xfy' size='340' side='right' caption='[[5xfy]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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<StructureSection load='5xfy' size='340' side='right'caption='[[5xfy]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5xfy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XFY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5xfy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ideonella_sakaiensis Ideonella sakaiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XFY FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xg0|5xg0]], [[5xfx|5xfx]], [[5xfz|5xfz]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(ethylene_terephthalate)_hydrolase Poly(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.101 3.1.1.101] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfy OCA], [https://pdbe.org/5xfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xfy RCSB], [https://www.ebi.ac.uk/pdbsum/5xfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfy ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfy OCA], [http://pdbe.org/5xfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xfy RCSB], [http://www.ebi.ac.uk/pdbsum/5xfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfy ProSAT]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PETH_IDESA PETH_IDESA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
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Structural insight into catalytic mechanism of PET hydrolase.,Han X, Liu W, Huang JW, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT Nat Commun. 2017 Dec 13;8(1):2106. doi: 10.1038/s41467-017-02255-z. PMID:29235460<ref>PMID:29235460</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5xfy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Chen, C C]]
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[[Category: Ideonella sakaiensis]]
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[[Category: Guo, R T]]
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[[Category: Large Structures]]
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[[Category: Han, X]]
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[[Category: Chen CC]]
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[[Category: Liu, W D]]
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[[Category: Guo RT]]
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[[Category: Zheng, Y Y]]
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[[Category: Han X]]
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[[Category: Acidocalcisomal pyrophosphatase]]
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[[Category: Liu WD]]
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[[Category: Hydrolase]]
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[[Category: Zheng YY]]
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[[Category: Inhibitor]]
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[[Category: Metal-binding]]
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[[Category: Substrate binding]]
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Current revision

Crystal structure of a novel PET hydrolase S131A mutant from Ideonella sakaiensis 201-F6

PDB ID 5xfy

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