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5xfz

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==Crystal structure of a novel PET hydrolase R103G/S131A mutant from Ideonella sakaiensis 201-F6==
==Crystal structure of a novel PET hydrolase R103G/S131A mutant from Ideonella sakaiensis 201-F6==
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<StructureSection load='5xfz' size='340' side='right' caption='[[5xfz]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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<StructureSection load='5xfz' size='340' side='right'caption='[[5xfz]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5xfz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ideonella_sakaiensis Ideonella sakaiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XFZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xg0|5xg0]], [[5xfx|5xfx]], [[5xfy|5xfy]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(ethylene_terephthalate)_hydrolase Poly(ethylene terephthalate) hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.101 3.1.1.101] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [https://pdbe.org/5xfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [https://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xfz OCA], [http://pdbe.org/5xfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xfz RCSB], [http://www.ebi.ac.uk/pdbsum/5xfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xfz ProSAT]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PETH_IDESA PETH_IDESA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
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Structural insight into catalytic mechanism of PET hydrolase.,Han X, Liu W, Huang JW, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT Nat Commun. 2017 Dec 13;8(1):2106. doi: 10.1038/s41467-017-02255-z. PMID:29235460<ref>PMID:29235460</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5xfz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Chen, C C]]
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[[Category: Ideonella sakaiensis]]
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[[Category: Guo, R T]]
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[[Category: Large Structures]]
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[[Category: Han, X]]
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[[Category: Chen CC]]
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[[Category: Liu, W D]]
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[[Category: Guo RT]]
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[[Category: Zheng, Y Y]]
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[[Category: Han X]]
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[[Category: Acidocalcisomal pyrophosphatase]]
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[[Category: Liu WD]]
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[[Category: Hydrolase]]
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[[Category: Zheng YY]]
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[[Category: Inhibitor]]
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[[Category: Metal-binding]]
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[[Category: Substrate binding]]
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Current revision

Crystal structure of a novel PET hydrolase R103G/S131A mutant from Ideonella sakaiensis 201-F6

PDB ID 5xfz

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