1tg7

From Proteopedia

(Difference between revisions)

Current revision

Native structure of beta-galactosidase from Penicillium sp.

Structural highlights

1tg7 is a 1 chain structure with sequence from Penicillium sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:	, , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BGALA_PENSQ Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Has high in vitro transglycosylation activity with p-nitrophenyl-beta-D-galactopyranoside, methyl-beta-D-galactopyranoside or lactose as a donor and galactose as an acceptor.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date.

Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.,Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:15491613^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Neustroev KN, de Sousa EA, Golubev AM, Brandão Neto, Eneyskaya EV, Kulminskaya AA, Polikarpov I. Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp. Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1508-9. PMID:11053867 doi:10.1107/s0907444900011756
↑ Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I. Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose. J Mol Biol. 2004 Nov 5;343(5):1281-92. PMID:15491613 doi:10.1016/j.jmb.2004.09.012

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tg7"

@@ Line 1: / Line 1: @@
 ==Native structure of beta-galactosidase from Penicillium sp.==
-<StructureSection load='1tg7' size='340' side='right' caption='[[1tg7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='1tg7' size='340' side='right'caption='[[1tg7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1tg7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Penicillium_sp. Penicillium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TG7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TG7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1tg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_sp. Penicillium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TG7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bgl|1bgl]], [[1kwg|1kwg]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tg7 OCA], [https://pdbe.org/1tg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1tg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tg7 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tg7 OCA], [http://pdbe.org/1tg7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tg7 RCSB], [http://www.ebi.ac.uk/pdbsum/1tg7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tg7 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/BGALA_PENSQ BGALA_PENSQ] Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Has high in vitro transglycosylation activity with p-nitrophenyl-beta-D-galactopyranoside, methyl-beta-D-galactopyranoside or lactose as a donor and galactose as an acceptor.<ref>PMID:11053867</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 14: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tg7_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 1tg7" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Galactosidase 3D structures|Galactosidase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Beta-galactosidase]]
+[[Category: Large Structures]]
 [[Category: Penicillium sp]]
-[[Category: Adamska, M]]
+[[Category: Adamska M]]
-[[Category: Arand, M]]
+[[Category: Arand M]]
-[[Category: Eneyskaya, E V]]
+[[Category: Eneyskaya EV]]
-[[Category: Garratt, R C]]
+[[Category: Garratt RC]]
-[[Category: Golubev, A M]]
+[[Category: Golubev AM]]
-[[Category: Kulminskaya, A A]]
+[[Category: Kulminskaya AA]]
-[[Category: Nagem, R A.P]]
+[[Category: Nagem RAP]]
-[[Category: Neustroev, K N]]
+[[Category: Neustroev KN]]
-[[Category: Polikarpov, I]]
+[[Category: Polikarpov I]]
-[[Category: Rojas, A L]]
+[[Category: Rojas AL]]
-[[Category: Family gh35]]
-[[Category: Glycoprotein]]
-[[Category: Glycoside hydrolase]]
-[[Category: Hydrolase]]
-[[Category: Penicillium]]
-[[Category: Tim barrel domain]]

1tg7

From Proteopedia

Current revision

Native structure of beta-galactosidase from Penicillium sp.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox