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4b5y

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==X-ray structure of the cyan fluorescent protein mTurquoise-GL (K206A mutant) in space group C222(1)==
==X-ray structure of the cyan fluorescent protein mTurquoise-GL (K206A mutant) in space group C222(1)==
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<StructureSection load='4b5y' size='340' side='right' caption='[[4b5y]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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<StructureSection load='4b5y' size='340' side='right'caption='[[4b5y]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4b5y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B5Y FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4b5y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B5Y FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b9c|1b9c]], [[1bfp|1bfp]], [[1c4f|1c4f]], [[1cv7|1cv7]], [[1ema|1ema]], [[1emb|1emb]], [[1emc|1emc]], [[1eme|1eme]], [[1emf|1emf]], [[1emg|1emg]], [[1emk|1emk]], [[1eml|1eml]], [[1emm|1emm]], [[1f09|1f09]], [[1f0b|1f0b]], [[1gfl|1gfl]], [[1h6r|1h6r]], [[1hcj|1hcj]], [[1huy|1huy]], [[1jby|1jby]], [[1jbz|1jbz]], [[1jc0|1jc0]], [[1jc1|1jc1]], [[1kp5|1kp5]], [[1kyp|1kyp]], [[1kyr|1kyr]], [[1kys|1kys]], [[1myw|1myw]], [[1q4a|1q4a]], [[1q4b|1q4b]], [[1q4c|1q4c]], [[1q4d|1q4d]], [[1q4e|1q4e]], [[1q73|1q73]], [[1qxt|1qxt]], [[1qy3|1qy3]], [[1qyf|1qyf]], [[1qyo|1qyo]], [[1qyq|1qyq]], [[1rm9|1rm9]], [[1rmm|1rmm]], [[1rmo|1rmo]], [[1rmp|1rmp]], [[1rrx|1rrx]], [[1w7s|1w7s]], [[1w7t|1w7t]], [[1w7u|1w7u]], [[1yfp|1yfp]], [[1yhg|1yhg]], [[1yhh|1yhh]], [[1yhi|1yhi]], [[1yj2|1yj2]], [[1yjf|1yjf]], [[1z1p|1z1p]], [[1z1q|1z1q]], [[2ah8|2ah8]], [[2aha|2aha]], [[2b3p|2b3p]], [[2b3q|2b3q]], [[2emd|2emd]], [[2emn|2emn]], [[2emo|2emo]], [[2fwq|2fwq]], [[2fzu|2fzu]], [[2wsn|2wsn]], [[2wso|2wso]], [[2wur|2wur]], [[2y0g|2y0g]], [[2ydz|2ydz]], [[2ye0|2ye0]], [[2ye1|2ye1]], [[2yfp|2yfp]], [[3ztf|3ztf]], [[4anj|4anj]], [[4ar7|4ar7]], [[4as8|4as8]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b5y OCA], [http://pdbe.org/4b5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b5y RCSB], [http://www.ebi.ac.uk/pdbsum/4b5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b5y ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b5y OCA], [https://pdbe.org/4b5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b5y RCSB], [https://www.ebi.ac.uk/pdbsum/4b5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b5y ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (&gt;3.7 ns) and high quantum yields (&gt;0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor.
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Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.,Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr Nat Methods. 2010 Feb;7(2):137-9. Epub 2010 Jan 17. PMID:20081836<ref>PMID:20081836</ref>
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4b5y" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aeqvi]]
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[[Category: Aequorea victoria]]
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[[Category: Gadella, T W.J]]
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[[Category: Large Structures]]
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[[Category: Goedhart, J]]
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[[Category: Gadella TWJ]]
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[[Category: Lelimousin, M]]
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[[Category: Goedhart J]]
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[[Category: Noirclerc-Savoye, M]]
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[[Category: Lelimousin M]]
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[[Category: Oost, K]]
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[[Category: Noirclerc-Savoye M]]
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[[Category: Royant, A]]
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[[Category: Oost K]]
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[[Category: Stetten, D von]]
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[[Category: Royant A]]
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[[Category: Cfp]]
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[[Category: Von Stetten D]]
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[[Category: Fluorescent protein]]
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[[Category: Fret]]
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[[Category: Fret donor]]
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Current revision

X-ray structure of the cyan fluorescent protein mTurquoise-GL (K206A mutant) in space group C222(1)

PDB ID 4b5y

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