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| | ==Crystal structure of a Francisella virulence factor FvfA in the hexagonal form== | | ==Crystal structure of a Francisella virulence factor FvfA in the hexagonal form== |
| - | <StructureSection load='5evf' size='340' side='right' caption='[[5evf]], [[Resolution|resolution]] 1.76Å' scene=''> | + | <StructureSection load='5evf' size='340' side='right'caption='[[5evf]], [[Resolution|resolution]] 1.76Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._novicida"_(larson_et_al._1955)_sjostedt_2005 "francisella tularensis subsp. novicida" (larson et al. 1955) sjostedt 2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EVF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EVF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.762Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5evg|5evg]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [https://pdbe.org/5evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [https://www.ebi.ac.uk/pdbsum/5evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACX55_1794 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [http://pdbe.org/5evf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [http://www.ebi.ac.uk/pdbsum/5evf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0Q625_FRATN A0Q625_FRATN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Craig, L]] | + | [[Category: Francisella tularensis subsp. novicida]] |
| - | [[Category: Guttman, J A]] | + | [[Category: Large Structures]] |
| - | [[Category: Kolappan, S]] | + | [[Category: Craig L]] |
| - | [[Category: Lo, K Y]] | + | [[Category: Guttman JA]] |
| - | [[Category: Shen, C L.J]] | + | [[Category: Kolappan S]] |
| - | [[Category: Francisella tularensis]] | + | [[Category: Lo KY]] |
| - | [[Category: Unknown function]] | + | [[Category: Shen CLJ]] |
| - | [[Category: Virulence factor]]
| + | |
| Structural highlights
Function
A0Q625_FRATN
Publication Abstract from PubMed
Francisella tularensis is a potent human pathogen that invades and survives in macrophage and epithelial cells. Two identical proteins, FTT_0924 from F. tularensis subsp. tularensis and FTL_1286 from F. tularensis subsp. holarctica LVS, have previously been identified as playing a role in protection of the bacteria from osmotic shock and its survival in macrophages. FTT_0924 has been shown to localize to the inner membrane, with its C-terminus exposed to the periplasm. Here, crystal structures of the F. novicida homologue FTN_0802, which we call FvfA, in two crystal forms are reported at 1.8 A resolution. FvfA differs from FTT_0924 and FTL_1286 by a single amino acid. FvfA has a DUF1471 fold that closely resembles the Escherichia coli outer membrane lipoprotein RscF, a component of a phosphorelay pathway involved in protecting bacteria from outer membrane perturbation. The structural and functional similarities and differences between these proteins and their implications for F. tularensis pathogenesis are discussed.
Structure of the conserved Francisella virulence protein FvfA.,Kolappan S, Lo KY, Shen CLJ, Guttman JA, Craig L Acta Crystallogr D Struct Biol. 2017 Oct 1;73(Pt 10):814-821. doi:, 10.1107/S205979831701333X. Epub 2017 Sep 27. PMID:28994410[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kolappan S, Lo KY, Shen CLJ, Guttman JA, Craig L. Structure of the conserved Francisella virulence protein FvfA. Acta Crystallogr D Struct Biol. 2017 Oct 1;73(Pt 10):814-821. doi:, 10.1107/S205979831701333X. Epub 2017 Sep 27. PMID:28994410 doi:http://dx.doi.org/10.1107/S205979831701333X
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