1sky

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[[Image:1sky.gif|left|200px]]<br />
 
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<applet load="1sky" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sky, resolution 3.2&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3==
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BACKGROUND: F1-ATPase, an oligomeric assembly with subunit stoichiometry, alpha 3 beta 3 gamma delta epsilon, is the catalytic component of the ATP, synthase complex, which plays a central role in energy transduction in, bacteria, chloroplasts and mitochondria. The crystal structure of bovine, mitochondrial F1-ATPase displays a marked asymmetry in the conformation, and nucleotide content of the catalytic beta subunits. The alpha 3 beta 3, subcomplex of F1-ATPase has been assembled from subunits of the moderately, thermophilic Bacillus PS3 made in Escherichia coli, and the subcomplex is, active but does not show the catalytic cooperativity of intact F1-ATPase., The structure of this subcomplex should provide new information on the, conformational variability of F1-ATPase and may provide insights into the, unusual catalytic mechanism employed by this enzyme. RESULTS: The crystal, structure of the nucleotide-free bacterial alpha 3 beta 3 subcomplex of, F1-ATPase, determined at 3.2 A resolution, shows that the oligomer has, exact threefold symmetry. The bacterial beta subunits adopt a conformation, essentially identical to that of the nucleotide-free beta subunit in, mitochondrial F1-ATPase; the alpha subunits have similar conformations in, both structures. CONCLUSIONS: The structures of the bacterial F1-ATPase, alpha and beta subunits are very similar to their counterparts in the, mitochondrial enzyme, suggesting a common catalytic mechanism. The study, presented here allows an analysis of the different conformations adopted, by the alpha and beta subunits and may ultimately further our, understanding of this mechanism.
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<StructureSection load='1sky' size='340' side='right'caption='[[1sky]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sky]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._PS3 Bacillus sp. PS3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sky OCA], [https://pdbe.org/1sky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sky RCSB], [https://www.ebi.ac.uk/pdbsum/1sky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sky ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ATPA_BACP3 ATPA_BACP3] Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.[HAMAP-Rule:MF_01346]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1sky_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sky ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp._ps3 Bacillus sp. ps3] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] Structure known Active Site: CAT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SKY OCA].
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*[[ATPase 3D structures|ATPase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer., Shirakihara Y, Leslie AG, Abrahams JP, Walker JE, Ueda T, Sekimoto Y, Kambara M, Saika K, Kagawa Y, Yoshida M, Structure. 1997 Jun 15;5(6):825-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9261073 9261073]
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[[Category: Bacillus sp. PS3]]
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[[Category: Bacillus sp. ps3]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Abrahams JP]]
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[[Category: Transferred entry: 3.6.3.14]]
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[[Category: Kagawa Y]]
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[[Category: Abrahams, J.P.]]
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[[Category: Kambara M]]
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[[Category: Kagawa, Y.]]
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[[Category: Leslie AGW]]
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[[Category: Kambara, M.]]
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[[Category: Saika K]]
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[[Category: Leslie, A.G.W.]]
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[[Category: Sekimoto Y]]
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[[Category: Saika, K.]]
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[[Category: Shirakihara Y]]
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[[Category: Sekimoto, Y.]]
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[[Category: Ueda T]]
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[[Category: Shirakihara, Y.]]
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[[Category: Walker JE]]
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[[Category: Ueda, T.]]
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[[Category: Yoshida M]]
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[[Category: Walker, J.E.]]
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[[Category: Yoshida, M.]]
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[[Category: SO4]]
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[[Category: alpha3beta3 subcomplex of f1-atpase]]
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[[Category: atp synthase]]
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[[Category: f1-atpase]]
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[[Category: f1fo atp synthase]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:04:39 2007''
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Current revision

CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3

PDB ID 1sky

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