5yvm

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of the archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase ADH/D1 bound to NZQ

Structural highlights

5yvm is a 1 chain structure with sequence from Candidate division MSBL1 archaeon SCGC-AAA259E19. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.12Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A133UP32_9EURY

Publication Abstract from PubMed

Because only 0.01% of prokaryotic genospecies can be cultured and in situ observations are often impracticable, culture-independent methods are required to understand microbial life and harness potential applications of microbes. Here, we report a methodology for the production of proteins with desired functions based on single amplified genomes (SAGs) from unculturable species. We use this method to resurrect an alcohol dehydrogenase (ADH/D1) from an uncharacterized halo-thermophilic archaeon collected from a brine pool at the bottom of the Red Sea. Our crystal structure of 5,6-dihydroxy NADPH-bound ADH/D1 combined with biochemical analyses reveal the molecular features of its halo-thermophily, its unique habitat adaptations, and its possible reaction mechanism for atypical oxygen activation. Our strategy offers a general guide for using SAGs as a source for scientific and industrial investigations of "microbial dark matter."

Identification and Experimental Characterization of an Extremophilic Brine Pool Alcohol Dehydrogenase from Single Amplified Genomes.,Grotzinger SW, Karan R, Strillinger E, Bader S, Frank A, Al Rowaihi IS, Akal A, Wackerow W, Archer JA, Rueping M, Weuster-Botz D, Groll M, Eppinger J, Arold ST ACS Chem Biol. 2017 Dec 18. doi: 10.1021/acschembio.7b00792. PMID:29188989^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grotzinger SW, Karan R, Strillinger E, Bader S, Frank A, Al Rowaihi IS, Akal A, Wackerow W, Archer JA, Rueping M, Weuster-Botz D, Groll M, Eppinger J, Arold ST. Identification and Experimental Characterization of an Extremophilic Brine Pool Alcohol Dehydrogenase from Single Amplified Genomes. ACS Chem Biol. 2017 Dec 18. doi: 10.1021/acschembio.7b00792. PMID:29188989 doi:http://dx.doi.org/10.1021/acschembio.7b00792

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5yvm"

@@ Line 1: / Line 1: @@
-==Crystal Structure of ADH/D1, an archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase==
+==Crystal Structure of the archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase ADH/D1 bound to NZQ==
-<StructureSection load='5yvm' size='340' side='right' caption='[[5yvm]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
+<StructureSection load='5yvm' size='340' side='right'caption='[[5yvm]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5yvm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YVM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YVM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5yvm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidate_division_MSBL1_archaeon_SCGC-AAA259E19 Candidate division MSBL1 archaeon SCGC-AAA259E19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YVM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NZQ:5,6-DIHYDROXY-NADP'>NZQ</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yvm OCA], [http://pdbe.org/5yvm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yvm RCSB], [http://www.ebi.ac.uk/pdbsum/5yvm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yvm ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NZQ:5,6-DIHYDROXY-NADP'>NZQ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yvm OCA], [https://pdbe.org/5yvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yvm RCSB], [https://www.ebi.ac.uk/pdbsum/5yvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yvm ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A133UP32_9EURY A0A133UP32_9EURY]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 19: @@
 </div>
 <div class="pdbe-citations 5yvm" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Arold, S T]]
+[[Category: Candidate division MSBL1 archaeon SCGC-AAA259E19]]
-[[Category: Eppinger, J]]
+[[Category: Large Structures]]
-[[Category: Frank, A]]
+[[Category: Arold ST]]
-[[Category: Groll, M]]
+[[Category: Eppinger J]]
-[[Category: Grotzinger, S W]]
+[[Category: Frank A]]
-[[Category: Strillinger, E]]
+[[Category: Groetzinger SW]]
-[[Category: Dehydrogenase]]
+[[Category: Groll M]]
-[[Category: Halophilic]]
+[[Category: Strillinger E]]
-[[Category: Nzq]]
-[[Category: Oxidoreductase]]
-[[Category: Protein-cofactor complex]]
-[[Category: Thermophilic]]

5yvm

From Proteopedia

Current revision

Crystal Structure of the archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase ADH/D1 bound to NZQ

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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