5yvs

==Crystal Structure of ADH/D1, an archaeal halo-thermophilic Red Sea brine pool alcohol dehydrogenase==

<StructureSection load='5yvs' size='340' side='right' caption='[[5yvs]], [[Resolution|resolution]] 2.34&Aring;' scene=''>

<table><tr><td colspan='2'>[[5yvs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YVS FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5yvm|5yvm]], [[5yvr|5yvr]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yvs OCA], [http://pdbe.org/5yvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yvs RCSB], [http://www.ebi.ac.uk/pdbsum/5yvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yvs ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Because only 0.01% of prokaryotic genospecies can be cultured and in situ observations are often impracticable, culture-independent methods are required to understand microbial life and harness potential applications of microbes. Here, we report a methodology for the production of proteins with desired functions based on single amplified genomes (SAGs) from unculturable species. We use this method to resurrect an alcohol dehydrogenase (ADH/D1) from an uncharacterized halo-thermophilic archaeon collected from a brine pool at the bottom of the Red Sea. Our crystal structure of 5,6-dihydroxy NADPH-bound ADH/D1 combined with biochemical analyses reveal the molecular features of its halo-thermophily, its unique habitat adaptations, and its possible reaction mechanism for atypical oxygen activation. Our strategy offers a general guide for using SAGs as a source for scientific and industrial investigations of "microbial dark matter."

 

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== References ==

[[Category: Arold, S T]]

[[Category: Eppinger, J]]

[[Category: Frank, A]]

[[Category: Groll, M]]

[[Category: Grotzinger, S W]]

[[Category: Strillinger, E]]

[[Category: Dehydrogenase]]

[[Category: Halophilic]]

[[Category: Thermophilic]]