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| | ==Crystal structure of the Arabidopsis thaliana chloroplast RNA editing factors 2(MORF2)== | | ==Crystal structure of the Arabidopsis thaliana chloroplast RNA editing factors 2(MORF2)== |
| - | <StructureSection load='5ydg' size='340' side='right' caption='[[5ydg]], [[Resolution|resolution]] 2.41Å' scene=''> | + | <StructureSection load='5ydg' size='340' side='right'caption='[[5ydg]], [[Resolution|resolution]] 2.41Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ydg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YDG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YDG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ydg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YDG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MORF2, DAL1, RIP2, At2g33430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.405Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ydg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ydg OCA], [http://pdbe.org/5ydg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ydg RCSB], [http://www.ebi.ac.uk/pdbsum/5ydg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ydg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ydg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ydg OCA], [https://pdbe.org/5ydg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ydg RCSB], [https://www.ebi.ac.uk/pdbsum/5ydg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ydg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MORF2_ARATH MORF2_ARATH]] Involved in plastid rRNA processing and consequently in translation and early chloroplast differentiation (PubMed:12678554). Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).<ref>PMID:12678554</ref> <ref>PMID:22411807</ref> <ref>PMID:23818871</ref> | + | [https://www.uniprot.org/uniprot/MORF2_ARATH MORF2_ARATH] Involved in plastid rRNA processing and consequently in translation and early chloroplast differentiation (PubMed:12678554). Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).<ref>PMID:12678554</ref> <ref>PMID:22411807</ref> <ref>PMID:23818871</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | RNA editing is a post-transcription process that alters the genetic information on RNA molecules. In plastids and mitochondria of flowering plants, the multiple organellar RNA editing factors (MORFs) interact with the PLS-type pentatricopeptide repeat (PPR) proteins and participate in RNA editing of cytidine-to-uridine conversion. The PPR proteins recognize cytidine targets around the editing sites, and the MORF proteins modulate the RNA-binding activity of the PPR proteins. Here, we report the structure of the Arabidopsis thaliana chloroplast MORF2 at 2.4 A resolution. The structure, adopting typical MORF-box fold as observed in mitochondrial MORF1 and chloroplast MORF9, reveals an MORF1-like dimerization mode. The difference between the two dimerization modes can be attributed to F157 (corresponding F162 in MORF1 and W160 in MORF9), which causes a 60 degrees shift upon dimerization. This observation, together with the PPR-MORF2 model, suggests a dimer-to-monomer transition during RNA editosome formation.
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| - | Crystal structure of the chloroplast RNA editing factor MORF2.,Yang J, Zhang M, Wang X Biochem Biophys Res Commun. 2017 Dec 8. pii: S0006-291X(17)32435-X. doi:, 10.1016/j.bbrc.2017.12.044. PMID:29229384<ref>PMID:29229384</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 5ydg" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | |
| - | [[Category: Gao, Y S]] | |
| - | [[Category: Wang, X]] | |
| - | [[Category: Wang, Y L]] | |
| - | [[Category: Yang, J Y]] | |
| | [[Category: Arabidopsis thaliana]] | | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Morf]] | + | [[Category: Large Structures]] |
| - | [[Category: Rna binding protein]] | + | [[Category: Gao YS]] |
| - | [[Category: Rna editing]] | + | [[Category: Wang X]] |
| | + | [[Category: Wang YL]] |
| | + | [[Category: Yang JY]] |
| Structural highlights
Function
MORF2_ARATH Involved in plastid rRNA processing and consequently in translation and early chloroplast differentiation (PubMed:12678554). Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).[1] [2] [3]
References
- ↑ Bisanz C, Begot L, Carol P, Perez P, Bligny M, Pesey H, Gallois JL, Lerbs-Mache S, Mache R. The Arabidopsis nuclear DAL gene encodes a chloroplast protein which is required for the maturation of the plastid ribosomal RNAs and is essential for chloroplast differentiation. Plant Mol Biol. 2003 Mar;51(5):651-63. PMID:12678554
- ↑ Takenaka M, Zehrmann A, Verbitskiy D, Kugelmann M, Hartel B, Brennicke A. Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5104-9. doi:, 10.1073/pnas.1202452109. Epub 2012 Mar 12. PMID:22411807 doi:http://dx.doi.org/10.1073/pnas.1202452109
- ↑ Bentolila S, Oh J, Hanson MR, Bukowski R. Comprehensive high-resolution analysis of the role of an Arabidopsis gene family in RNA editing. PLoS Genet. 2013 Jun;9(6):e1003584. doi: 10.1371/journal.pgen.1003584. Epub 2013 , Jun 20. PMID:23818871 doi:http://dx.doi.org/10.1371/journal.pgen.1003584
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