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| | ==Structure of UreE== | | ==Structure of UreE== |
| - | <StructureSection load='1gmu' size='340' side='right' caption='[[1gmu]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='1gmu' size='340' side='right'caption='[[1gmu]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1gmu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GMU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GMU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1gmu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GMU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1gmv|1gmv]], [[1gmw|1gmw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gmu OCA], [http://pdbe.org/1gmu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gmu RCSB], [http://www.ebi.ac.uk/pdbsum/1gmu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gmu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gmu OCA], [https://pdbe.org/1gmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gmu RCSB], [https://www.ebi.ac.uk/pdbsum/1gmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gmu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UREE_ENTAE UREE_ENTAE]] Involved in urease metallocenter assembly. Binds about 6 nickel ions per homodimer. Probably functions as a nickel donor during metallocenter assembly. Its function can be bypassed in vitro in the presence of high nickel concentrations. | + | [https://www.uniprot.org/uniprot/UREE_KLEAE UREE_KLEAE] Involved in urease metallocenter assembly. Binds about 6 nickel ions per homodimer. Probably functions as a nickel donor during metallocenter assembly. Its function can be bypassed in vitro in the presence of high nickel concentrations.[HAMAP-Rule:MF_00822] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Urease accessory protein|Urease accessory protein]] | + | *[[Urease accessory protein 3D structures|Urease accessory protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hausinger, R]] | + | [[Category: Klebsiella aerogenes]] |
| - | [[Category: Huber, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Mulrooney, S B]] | + | [[Category: Hausinger R]] |
| - | [[Category: Song, H K]] | + | [[Category: Huber R]] |
| - | [[Category: Metallochaperone]] | + | [[Category: Mulrooney SB]] |
| | + | [[Category: Song HK]] |
| Structural highlights
Function
UREE_KLEAE Involved in urease metallocenter assembly. Binds about 6 nickel ions per homodimer. Probably functions as a nickel donor during metallocenter assembly. Its function can be bypassed in vitro in the presence of high nickel concentrations.[HAMAP-Rule:MF_00822]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
UreE is proposed to be a metallochaperone that delivers nickel ions to urease during activation of this bacterial virulence factor. Wild-type Klebsiella aerogenes UreE binds approximately six nickel ions per homodimer, whereas H144*UreE (a functional C-terminal truncated variant) was previously reported to bind two. We determined the structure of H144*UreE by multi-wavelength anomalous diffraction and refined it to 1.5 A resolution. The present structure reveals an Hsp40-like peptide-binding domain, an Atx1-like metal-binding domain, and a flexible C terminus. Three metal-binding sites per dimer, defined by structural analysis of Cu-H144*UreE, are on the opposite face of the Atx1-like domain than observed in the copper metallochaperone. One metal bridges the two subunits via the pair of His-96 residues, whereas the other two sites involve metal coordination by His-110 and His-112 within each subunit. In contrast to the copper metallochaperone mechanism involving thiol ligand exchanges between structurally similar chaperones and target proteins, we propose that the Hsp40-like module interacts with urease apoprotein and/or other urease accessory proteins, while the Atx1-like domain delivers histidyl-bound nickel to the urease active site.
Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation.,Song HK, Mulrooney SB, Huber R, Hausinger RP J Biol Chem. 2001 Dec 28;276(52):49359-64. Epub 2001 Oct 8. PMID:11591723[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Song HK, Mulrooney SB, Huber R, Hausinger RP. Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation. J Biol Chem. 2001 Dec 28;276(52):49359-64. Epub 2001 Oct 8. PMID:11591723 doi:10.1074/jbc.M108619200
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