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2czu

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[[Image:2czu.gif|left|200px]]
 
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{{Structure
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==lipocalin-type prostaglandin D synthase==
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|PDB= 2czu |SIZE=350|CAPTION= <scene name='initialview01'>2czu</scene>, resolution 2.10&Aring;
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<StructureSection load='2czu' size='340' side='right'caption='[[2czu]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2czu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CZU FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2czu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czu OCA], [https://pdbe.org/2czu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2czu RCSB], [https://www.ebi.ac.uk/pdbsum/2czu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2czu ProSAT], [https://www.topsan.org/Proteins/RSGI/2czu TOPSAN]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2czt|2CZT]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2czu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czu OCA], [http://www.ebi.ac.uk/pdbsum/2czu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2czu RCSB]</span>
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[https://www.uniprot.org/uniprot/PTGDS_MOUSE PTGDS_MOUSE] Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.<ref>PMID:8922532</ref> <ref>PMID:9892701</ref> <ref>PMID:10781097</ref> <ref>PMID:11751991</ref> <ref>PMID:12077186</ref> <ref>PMID:17715133</ref> <ref>PMID:19546224</ref> <ref>PMID:19833210</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cz/2czu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2czu ConSurf].
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<div style="clear:both"></div>
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'''lipocalin-type prostaglandin D synthase'''
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==See Also==
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*[[Prostaglandin D synthase|Prostaglandin D synthase]]
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== References ==
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==Overview==
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<references/>
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Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2CZU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZU OCA].
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==Reference==
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Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12761195 12761195]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Prostaglandin-D synthase]]
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[[Category: Ago H]]
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[[Category: Single protein]]
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[[Category: Aritake K]]
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[[Category: Ago, H.]]
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[[Category: Hayaishi O]]
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[[Category: Aritake, K.]]
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[[Category: Inoue T]]
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[[Category: Hayaishi, O.]]
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[[Category: Irikura D]]
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[[Category: Inoue, T.]]
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[[Category: Kumasaka T]]
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[[Category: Irikura, D.]]
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[[Category: Miyano M]]
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[[Category: Kumasaka, T.]]
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[[Category: Urade Y]]
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[[Category: Miyano, M.]]
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[[Category: Yamamoto M]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Urade, Y.]]
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[[Category: Yamamoto, M.]]
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[[Category: lipocalin]]
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[[Category: lpgds_p212121 native]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:28:27 2008''
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Current revision

lipocalin-type prostaglandin D synthase

PDB ID 2czu

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