5z38
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of CsrA bound to CesT== | |
| + | <StructureSection load='5z38' size='340' side='right'caption='[[5z38]], [[Resolution|resolution]] 2.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5z38]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_O127:H6_str._E2348/69 Escherichia coli O127:H6 str. E2348/69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z38 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.292Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z38 OCA], [https://pdbe.org/5z38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z38 RCSB], [https://www.ebi.ac.uk/pdbsum/5z38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z38 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CSRA_ECOLI CSRA_ECOLI] Binds to mRNA to regulate post-transcriptional activity. Affects glycogen biosynthesis, gluconeogenesis, cell size and surface properties. Regulates glycogen synthesis under both aerobic and anaerobic conditions. Seems to accelerate the degradation of glg gene transcripts, potentially through selective RNA binding. Acts to inhibit interaction between the LetD protein and the A subunit of DNA gyrase. Also required for motility and flagellum biosynthesis through the post-transcriptional activation of flhDC expression. This involves binding to and stabilization of the flhDC message by CsrA. Binds to and reduces levels of probable diguanylate cyclases ycdT and ydeH.<ref>PMID:11298291</ref> <ref>PMID:18713317</ref> <ref>PMID:19460094</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The T3SS chaperone CesT is recently shown to interact with the post-transcriptional regulator CsrA to modulate post-attachment signaling in enteropathogenic and enterohemorrhagic Escherichia coli. The molecular basis of the CesT/CsrA binding, however, remains elusive. Here, we show that CesT and CsrA both created two ligand binding sites in their homodimers, forming irregular multimeric complexes in solution. Through construction of a recombinant CsrA-dimer (Re-CsrA) that contains a single CesT binding site, the atomic binding features between CesT and CsrA are delineated via the structure of the CesT/Re-CsrA complex. In contrast to a previously reported N-terminally swapped dimer-form, CesT adopts a dimeric architecture with a swapped C-terminal helix for CsrA engagement. In CsrA, CesT binds to a surface patch that extensively overlaps with its mRNA binding site. The binding mode therefore justifies a mechanism of CsrA-modulation by CesT via competitive inhibition of the CsrA/mRNA interactions. | ||
| - | + | Molecular basis of binding between the global post-transcriptional regulator CsrA and the T3SS chaperone CesT.,Ye F, Yang F, Yu R, Lin X, Qi J, Chen Z, Cao Y, Wei Y, Gao GF, Lu G Nat Commun. 2018 Mar 22;9(1):1196. doi: 10.1038/s41467-018-03625-x. PMID:29567971<ref>PMID:29567971</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5z38" style="background-color:#fffaf0;"></div> |
| - | [[Category: Gao | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Yang | + | [[Category: Escherichia coli]] |
| - | [[Category: | + | [[Category: Escherichia coli O127:H6 str. E2348/69]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Chen Z]] | ||
| + | [[Category: Gao GF]] | ||
| + | [[Category: Lin X]] | ||
| + | [[Category: Lu G]] | ||
| + | [[Category: Qi J]] | ||
| + | [[Category: Yang F]] | ||
| + | [[Category: Ye F]] | ||
| + | [[Category: Yu R]] | ||
Current revision
Crystal structure of CsrA bound to CesT
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Categories: Escherichia coli | Escherichia coli O127:H6 str. E2348/69 | Large Structures | Chen Z | Gao GF | Lin X | Lu G | Qi J | Yang F | Ye F | Yu R
