1umx

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[[Image:1umx.gif|left|200px]]<br />
 
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<applet load="1umx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1umx, resolution 2.8&Aring;" />
 
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'''PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ARG M267 REPLACED WITH LEU (CHAIN M, R267L)'''<br />
 
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==Overview==
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==PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ARG M267 REPLACED WITH LEU (CHAIN M, R267L)==
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Relatively little is known about the functions of specific molecular, interactions between membrane proteins and membrane lipids. The structural, and functional consequences of disrupting a previously identified, interaction between a molecule of the diacidic lipid cardiolipin and the, purple bacterial reaction centre were examined. Mutagenesis of a highly, conserved arginine (M267) that is responsible for binding the head-group, of the cardiolipin (to leucine) did not affect the rate of photosynthetic, growth, the functional properties of the reaction centre, or the X-ray, crystal structure of the complex (determined to a resolution of 2.8 A)., However, the thermal stability of the protein was compromised by this, mutation, part of the reaction centre population showing an approximately, 5 degrees C decrease in melting temperature in response to the arginine to, leucine mutation. The crystallised mutant reaction centre also no longer, bound detectable amounts of cardiolipin at this site. Taken together, these observations suggest that this particular protein-lipid interaction, contributes to the thermal stability of the complex, at least when in, detergent micelles. These findings are discussed in the light of proposals, concerning the unfolding processes that occur when membrane proteins are, heated, and we propose that one function of the cardiolipin is to, stabilise the interaction between adjacent membrane-spanning alpha-helices, in a region where there are no direct protein-protein interactions.
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<StructureSection load='1umx' size='340' side='right'caption='[[1umx]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1umx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UMX FirstGlance]. <br>
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1UMX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE, PO4, LDA, BCL, BPB, SPN and U10 as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UMX OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1umx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umx OCA], [https://pdbe.org/1umx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1umx RCSB], [https://www.ebi.ac.uk/pdbsum/1umx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1umx ProSAT]</span></td></tr>
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Disruption of a specific molecular interaction with a bound lipid affects the thermal stability of the purple bacterial reaction centre., Fyfe PK, Isaacs NW, Cogdell RJ, Jones MR, Biochim Biophys Acta. 2004 Jan 30;1608(1):11-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14741581 14741581]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Rhodobacter sphaeroides]]
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[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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[[Category: Cogdell, R.J.]]
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== Evolutionary Conservation ==
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[[Category: Fyfe, P.K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Isaacs, N.W.]]
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Check<jmol>
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[[Category: Jones, M.R.]]
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<jmolCheckbox>
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[[Category: BCL]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/um/1umx_consurf.spt"</scriptWhenChecked>
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[[Category: BPB]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: FE]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: LDA]]
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</jmolCheckbox>
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[[Category: PO4]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1umx ConSurf].
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[[Category: SPN]]
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<div style="clear:both"></div>
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[[Category: U10]]
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__TOC__
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[[Category: cardiolipin]]
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</StructureSection>
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[[Category: electron transport]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: membrane protein]]
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[[Category: Large Structures]]
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[[Category: photosynthesis]]
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[[Category: Cogdell RJ]]
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[[Category: photosynthetic reaction center]]
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[[Category: Fyfe PK]]
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[[Category: transmembrane]]
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[[Category: Isaacs NW]]
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[[Category: Jones MR]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:06:42 2007''
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Current revision

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ARG M267 REPLACED WITH LEU (CHAIN M, R267L)

PDB ID 1umx

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