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| | ==Crystal structure of monomeric guanylyl cyclase domain of RhoGC fusion protein from the aquatic fungus Blastocladiella emersonii== | | ==Crystal structure of monomeric guanylyl cyclase domain of RhoGC fusion protein from the aquatic fungus Blastocladiella emersonii== |
| - | <StructureSection load='6ao9' size='340' side='right' caption='[[6ao9]], [[Resolution|resolution]] 1.13Å' scene=''> | + | <StructureSection load='6ao9' size='340' side='right'caption='[[6ao9]], [[Resolution|resolution]] 1.13Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ao9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquatic_fungus Aquatic fungus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AO9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AO9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ao9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastocladiella_emersonii Blastocladiella emersonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AO9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4808 Aquatic fungus])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.13Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ao9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ao9 OCA], [http://pdbe.org/6ao9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ao9 RCSB], [http://www.ebi.ac.uk/pdbsum/6ao9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ao9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ao9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ao9 OCA], [https://pdbe.org/6ao9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ao9 RCSB], [https://www.ebi.ac.uk/pdbsum/6ao9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ao9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A060H1D7_BLAEM A0A060H1D7_BLAEM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aquatic fungus]] | + | [[Category: Blastocladiella emersonii]] |
| - | [[Category: Kumar, R Prem]] | + | [[Category: Large Structures]] |
| - | [[Category: Oprian, D D]] | + | [[Category: Oprian DD]] |
| - | [[Category: Class iii nucleotidyl cyclase]] | + | [[Category: Prem Kumar R]] |
| - | [[Category: Enzyme]]
| + | |
| - | [[Category: Gc]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Nucleotide cyclase]]
| + | |
| - | [[Category: Rhogc]]
| + | |
| Structural highlights
Function
A0A060H1D7_BLAEM
Publication Abstract from PubMed
RhoGC is a fusion protein from the aquatic fungus Blastocladiella emersonii, combining a type I rhodopsin domain with a guanylyl cyclase domain. It has generated excitement as an optogenetics tool for the manipulation of cyclic nucleotide signaling pathways. To investigate the regulation of the cyclase activity, we isolated the guanylyl cyclase domain from Escherichia coli with (GCwCCRho) and without (GCRho) the coiled-coil (CC) linker. Both constructs were constitutively active but were monomeric as determined by size-exclusion chromatography and analytical ultracentrifugation, whereas other class III nucleotidyl cyclases are functional dimers. We also observed that crystals of GCRho have only a monomer in an asymmetric unit. Dimers formed when crystals were grown in the presence of the noncyclizable substrate analog 2',3'-dideoxyguanosine-5'-triphosphate (ddGTP), MnCl2, and tartrate, but their quaternary structure did not conform to the canonical pairing expected for class III enzymes. Moreover, the structure contained a disulfide bond formed with an active-site Cys residue required for activity. We consider it unlikely that the disulfide would form under intracellular reducing conditions, raising the possibility that this unusual dimer might have a biologically relevant role in the regulation of full-length RhoGC. While we did not observe it with direct methods, a functional dimer was identified as the active state by following the dependence of activity on total enzyme concentration. The low affinity observed for GCRho monomers is unusual for this enzyme class and suggests that dimer formation may contribute to light activation of the full-length protein.
Structure and monomer/dimer equilibrium for the guanylyl cyclase domain of the optogenetics protein RhoGC.,Kumar RP, Morehouse BR, Fofana J, Trieu MM, Zhou DH, Lorenz MO, Oprian DD J Biol Chem. 2017 Nov 8. pii: jbc.M117.812685. doi: 10.1074/jbc.M117.812685. PMID:29118188[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kumar RP, Morehouse BR, Fofana J, Trieu MM, Zhou DH, Lorenz MO, Oprian DD. Structure and monomer/dimer equilibrium for the guanylyl cyclase domain of the optogenetics protein RhoGC. J Biol Chem. 2017 Nov 8. pii: jbc.M117.812685. doi: 10.1074/jbc.M117.812685. PMID:29118188 doi:http://dx.doi.org/10.1074/jbc.M117.812685
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