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2d5x

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[[Image:2d5x.gif|left|200px]]
 
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{{Structure
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==Crystal structure of carbonmonoxy horse hemoglobin complexed with L35==
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|PDB= 2d5x |SIZE=350|CAPTION= <scene name='initialview01'>2d5x</scene>, resolution 1.45&Aring;
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<StructureSection load='2d5x' size='340' side='right'caption='[[2d5x]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=L35:2-[4-({[(3,5-DICHLOROPHENYL)AMINO]CARBONYL}AMINO)PHENOXY]-2-METHYLPROPANOIC+ACID'>L35</scene>
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<table><tr><td colspan='2'>[[2d5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D5X FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=L35:2-[4-({[(3,5-DICHLOROPHENYL)AMINO]CARBONYL}AMINO)PHENOXY]-2-METHYLPROPANOIC+ACID'>L35</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d5x OCA], [https://pdbe.org/2d5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d5x RCSB], [https://www.ebi.ac.uk/pdbsum/2d5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d5x ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2d5z|2D5Z]], [[2d60|2D60]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d5x OCA], [http://www.ebi.ac.uk/pdbsum/2d5x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2d5x RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/HBA_HORSE HBA_HORSE] Involved in oxygen transport from the lung to the various peripheral tissues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d5/2d5x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d5x ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of carbonmonoxy horse hemoglobin complexed with L35'''
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==See Also==
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Although detailed crystal structures of haemoglobin (Hb) provide a clear understanding of the basic allosteric mechanism of the protein, and how this in turn controls oxygen affinity, recent experiments with artificial effector molecules have shown a far greater control of oxygen binding than with natural heterotropic effectors. Contrary to the established text-book view, these non-physiological compounds are able to reduce oxygen affinity very strongly without switching the protein to the T (tense) state. In an earlier paper we showed that bezafibrate (BZF) binds to a surface pocket on the alpha subunits of R state Hb, strongly reducing the oxygen affinity of this protein conformation. Here we report the crystallisation of Hb with L35, a related compound, and show that this binds to the central cavity of both R and T state Hb. The mechanism by which L35 reduces oxygen affinity is discussed, in relation to spectroscopic studies of effector binding.
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==About this Structure==
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2D5X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5X OCA].
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==Reference==
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R-state haemoglobin with low oxygen affinity: crystal structures of deoxy human and carbonmonoxy horse haemoglobin bound to the effector molecule L35., Yokoyama T, Neya S, Tsuneshige A, Yonetani T, Park SY, Tame JR, J Mol Biol. 2006 Feb 24;356(3):790-801. Epub 2005 Dec 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16403522 16403522]
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[[Category: Equus caballus]]
[[Category: Equus caballus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Neya, S.]]
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[[Category: Neya S]]
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[[Category: Park, S Y.]]
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[[Category: Park SY]]
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[[Category: Tame, J R.]]
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[[Category: Tame JR]]
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[[Category: Tsuneshige, A.]]
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[[Category: Tsuneshige A]]
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[[Category: Yokoyama, T.]]
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[[Category: Yokoyama T]]
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[[Category: Yonetani, T.]]
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[[Category: Yonetani T]]
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[[Category: allosteric effector]]
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[[Category: crystal structure]]
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[[Category: hemoglobin]]
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[[Category: l35]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:30:41 2008''
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Current revision

Crystal structure of carbonmonoxy horse hemoglobin complexed with L35

PDB ID 2d5x

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