2d6f

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[[Image:2d6f.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)==
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|PDB= 2d6f |SIZE=350|CAPTION= <scene name='initialview01'>2d6f</scene>, resolution 3.15&Aring;
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<StructureSection load='2d6f' size='340' side='right'caption='[[2d6f]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[2d6f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D6F FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d6f OCA], [https://pdbe.org/2d6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d6f RCSB], [https://www.ebi.ac.uk/pdbsum/2d6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d6f ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d6f OCA], [http://www.ebi.ac.uk/pdbsum/2d6f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2d6f RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/GATD_METTH GATD_METTH] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d6/2d6f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d6f ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)'''
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==See Also==
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*[[Glutamyl-tRNA(Gln) amidotransferase|Glutamyl-tRNA(Gln) amidotransferase]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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==Overview==
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__TOC__
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Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code.
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</StructureSection>
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[[Category: Large Structures]]
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==About this Structure==
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2D6F is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D6F OCA].
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==Reference==
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Structural basis of RNA-dependent recruitment of glutamine to the genetic code., Oshikane H, Sheppard K, Fukai S, Nakamura Y, Ishitani R, Numata T, Sherrer RL, Feng L, Schmitt E, Panvert M, Blanquet S, Mechulam Y, Soll D, Nureki O, Science. 2006 Jun 30;312(5782):1950-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16809540 16809540]
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[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Methanothermobacter thermautotrophicus]]
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[[Category: Protein complex]]
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[[Category: Nureki O]]
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[[Category: Nureki, O.]]
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[[Category: ligase]]
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[[Category: rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:30:50 2008''
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Current revision

Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)

PDB ID 2d6f

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