2d7h

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[[Image:2d7h.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the ccc complex of the N-terminal domain of PriA==
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|PDB= 2d7h |SIZE=350|CAPTION= <scene name='initialview01'>2d7h</scene>, resolution 3.0&Aring;
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<StructureSection load='2d7h' size='340' side='right'caption='[[2d7h]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DCM:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DCM</scene>
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<table><tr><td colspan='2'>[[2d7h]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D7H FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d7h OCA], [https://pdbe.org/2d7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d7h RCSB], [https://www.ebi.ac.uk/pdbsum/2d7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d7h ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2d7g|2D7G]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d7h OCA], [http://www.ebi.ac.uk/pdbsum/2d7h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2d7h RCSB]</span>
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[https://www.uniprot.org/uniprot/PRIA_ECOLI PRIA_ECOLI] Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA. Is also involved in initiation of normal DNA replication in various plasmids and phages. Binds to branched DNA structures that resemble D-loops or to the primosome assembly site (PAS). Binds to DNA in two distinct modes, either dependent on or independent of the 3' terminus recognition.<ref>PMID:2825188</ref> <ref>PMID:8366072</ref> <ref>PMID:10356325</ref> <ref>PMID:10956036</ref> <ref>PMID:11929519</ref> <ref>PMID:12622722</ref> <ref>PMID:12917421</ref> <ref>PMID:15576682</ref> <ref>PMID:17483094</ref> <ref>PMID:23264623</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/2d7h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d7h ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the ccc complex of the N-terminal domain of PriA'''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Overview==
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<references/>
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PriA, a DEXH-type DNA helicase, binds specifically to the 3' end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3' terminus of DNA.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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2D7H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7H OCA].
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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==Reference==
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[[Category: Kohda D]]
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Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli., Sasaki K, Ose T, Tanaka T, Mizukoshi T, Ishigaki T, Maenaka K, Masai H, Kohda D, Biochim Biophys Acta. 2006 Jan;1764(1):157-60. Epub 2005 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16226927 16226927]
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[[Category: Maenaka K]]
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[[Category: Escherichia coli]]
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[[Category: Masai H]]
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[[Category: Single protein]]
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[[Category: Ose T]]
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[[Category: Kohda, D.]]
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[[Category: Sasaki K]]
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[[Category: Maenaka, K.]]
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[[Category: Masai, H.]]
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[[Category: Ose, T.]]
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[[Category: Sasaki, K.]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:31:02 2008''
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Current revision

Crystal structure of the ccc complex of the N-terminal domain of PriA

PDB ID 2d7h

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