5z3g
From Proteopedia
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(New page: '''Unreleased structure''' The entry 5z3g is ON HOLD Authors: Description: Category: Unreleased Structures) |
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- | '''Unreleased structure''' | ||
- | + | ==Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)== | |
+ | <SX load='5z3g' size='340' side='right' viewer='molstar' caption='[[5z3g]], [[Resolution|resolution]] 3.65Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[5z3g]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z3G FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.65Å</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3g OCA], [https://pdbe.org/5z3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z3g RCSB], [https://www.ebi.ac.uk/pdbsum/5z3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3g ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/RL6A_YEAST RL6A_YEAST] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 A resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits. | ||
- | + | Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.,Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K Protein Cell. 2018 Mar 19. pii: 10.1007/s13238-018-0526-7. doi:, 10.1007/s13238-018-0526-7. PMID:29557065<ref>PMID:29557065</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 5z3g" style="background-color:#fffaf0;"></div> | ||
+ | |||
+ | ==See Also== | ||
+ | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] | ||
+ | *[[Helicase 3D structures|Helicase 3D structures]] | ||
+ | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </SX> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Saccharomyces cerevisiae]] | ||
+ | [[Category: Saccharomyces cerevisiae S288C]] | ||
+ | [[Category: Ye K]] | ||
+ | [[Category: Zhou D]] | ||
+ | [[Category: Zhu X]] |
Current revision
Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
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