5z4g
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of secretory abundant heat soluble protein 4 from Ramazzottius varieornatus== | |
| + | <StructureSection load='5z4g' size='340' side='right'caption='[[5z4g]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5z4g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ramazzottius_varieornatus Ramazzottius varieornatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z4G FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z4g OCA], [https://pdbe.org/5z4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z4g RCSB], [https://www.ebi.ac.uk/pdbsum/5z4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z4g ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1D1UN89_RAMVA A0A1D1UN89_RAMVA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Though anhydrobiotic tardigrades (micro-animals also known as water bears) possess many genes of secretory abundant heat soluble (SAHS) proteins unique to Tardigrada, their functions are unknown. A previous crystallographic study revealed that a SAHS protein (RvSAHS1) from one of the toughest tardigrades, Ramazzottius varieornatus, has a beta-barrel architecture similar to fatty acid binding proteins (FABPs) and two putative ligand binding sites (LBS1 and LBS2) where fatty acids can bind. However, some SAHS proteins such as RvSAHS4 have different sets of amino acid residues at LBS1 and LBS2, implying that they prefer other ligands and have different functions. Here RvSAHS4 was crystallized and analyzed under a condition similar to that for RvSAHS1. There was no electron density corresponding to a fatty acid at LBS1 of RvSAHS4, where a putative fatty acid was observed in RvSAHS1. Instead, LBS2 of RvSAHS4, which was composed of uncharged residues, captured a putative polyethylene glycol molecule. These results suggest that RvSAHS4 mainly uses LBS2 for the binding of uncharged molecules. | ||
| - | + | Crystal structure of secretory abundant heat soluble protein 4 from one of the toughest "water bears" micro-animals Ramazzottius Varieornatus.,Fukuda Y, Inoue T Protein Sci. 2018 Mar 1. doi: 10.1002/pro.3393. PMID:29493034<ref>PMID:29493034</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5z4g" style="background-color:#fffaf0;"></div> |
| - | [[Category: Fukuda | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ramazzottius varieornatus]] | ||
| + | [[Category: Fukuda Y]] | ||
| + | [[Category: Inoue T]] | ||
Current revision
Crystal structure of secretory abundant heat soluble protein 4 from Ramazzottius varieornatus
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