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| | ==Crystal structure of the Variable Domain of the Streptococcus gordonii Surface Protein SspB== | | ==Crystal structure of the Variable Domain of the Streptococcus gordonii Surface Protein SspB== |
| - | <StructureSection load='2wd6' size='340' side='right' caption='[[2wd6]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='2wd6' size='340' side='right'caption='[[2wd6]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2wd6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WD6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2wd6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WD6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wd6 OCA], [http://pdbe.org/2wd6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wd6 RCSB], [http://www.ebi.ac.uk/pdbsum/2wd6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wd6 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wd6 OCA], [https://pdbe.org/2wd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wd6 RCSB], [https://www.ebi.ac.uk/pdbsum/2wd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wd6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SSP5_STRGN SSP5_STRGN]] May bind sialic acid residues of salivary agglutinin (SAG) in a calcium-dependent reaction. The interaction of SAG with its receptor in various oral streptococci modulate bacterial colonization of oral tissue and is associated with reduced levels of dental caries. | + | [https://www.uniprot.org/uniprot/SSPB_STRGN SSPB_STRGN] May bind sialic acid residues of salivary agglutinin (SAG) in a calcium-dependent reaction. The interaction of SAG with its receptor in various oral streptococci modulate bacterial colonization of oral tissue and is associated with reduced levels of dental caries. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 10558]] | + | [[Category: Large Structures]] |
| - | [[Category: Forsgren, N]] | + | [[Category: Streptococcus gordonii]] |
| - | [[Category: Persson, K]] | + | [[Category: Forsgren N]] |
| - | [[Category: Ag i/ii protein]] | + | [[Category: Persson K]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Cell wall]]
| + | |
| - | [[Category: Peptidoglycan-anchor]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Surface adhesin]]
| + | |
| - | [[Category: V-region]]
| + | |
| Structural highlights
Function
SSPB_STRGN May bind sialic acid residues of salivary agglutinin (SAG) in a calcium-dependent reaction. The interaction of SAG with its receptor in various oral streptococci modulate bacterial colonization of oral tissue and is associated with reduced levels of dental caries.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Antigen I/II (AgI/II) family of proteins are cell wall anchored adhesins expressed on the surface of oral streptococci. The AgI/II proteins interact with molecules on other bacteria, on the surface of host cells, and with salivary proteins. Streptococcus gordonii is a commensal bacterium, and one of the primary colonizers that initiate the formation of the oral biofilm. S. gordonii expresses two AgI/II proteins, SspA and SspB that are closely related. One of the domains of SspB, called the variable (V-) domain, is significantly different from corresponding domains in SspA and all other AgI/II proteins. As a first step to elucidate the differences among these proteins, we have determined the crystal structure of the V-domain from S. gordonii SspB at 2.3 A resolution. The domain comprises a betabeta-supersandwich with a putative binding cleft stabilized by a metal ion. The overall structure of the SspB V-domain is similar to the previously reported V-domain of the Streptococcus mutans protein SpaP, despite their low sequence similarity. In spite of the conserved architecture of the binding cleft, the cavity is significantly smaller in SspB which may provide clues about the difference in ligand specificity. We also verified that the metal in the binding cleft is a calcium ion, in concurrence with previous biological data. It was previously suggested that AgI/II V-domains are carbohydrate binding. However, we tested that hypothesis by screening the SspB V-domain for binding to over 400 glycoconjucates and found that the domain does not interact with any of the carbohydrates.
Crystal structure of the variable domain of the Streptococcus gordonii surface protein SspB.,Forsgren N, Lamont RJ, Persson K Protein Sci. 2009 Jul 16. PMID:19609934[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Forsgren N, Lamont RJ, Persson K. Crystal structure of the variable domain of the Streptococcus gordonii surface protein SspB. Protein Sci. 2009 Jul 16. PMID:19609934 doi:10.1002/pro.200
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