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| - | [[Image:2dfw.gif|left|200px]] | + | #REDIRECT [[3if5]] This PDB entry is obsolete and replaced by 3if5 |
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| - | {{Structure
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| - | |PDB= 2dfw |SIZE=350|CAPTION= <scene name='initialview01'>2dfw</scene>, resolution 2.44Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span>
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| - | |GENE=
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| - | |DOMAIN=
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| - | |RELATEDENTRY=
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dfw OCA], [http://www.ebi.ac.uk/pdbsum/2dfw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dfw RCSB]</span>
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| - | }}
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| - | '''Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3'''
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| - | ==Overview==
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| - | Glutaminase of Micrococcus luteus K-3 (intact glutaminase; 48kDa) is digested to a C-terminally truncated fragment (glutaminase fragment; 42kDa) that shows higher salt tolerance than that of the intact glutaminase. The crystal structure of the glutaminase fragment was determined at 2.4A resolution using multiple-wavelength anomalous dispersion (MAD). The glutaminase fragment is composed of N-terminal and C-terminal domains, and a putative catalytic serine-lysine dyad (S64 and K67) is located in a cleft of the N-terminal domain. Mutations of the S64 or K67 residues abolished the enzyme activity. The N-terminal domain has abundant glutamic acid residues on its surface, which may explain its salt-tolerant mechanism. A diffraction analysis of the intact glutaminase crystals (a twinning fraction of 0.43) located the glutaminase fragment in the unit cell but failed to turn up clear densities for the missing C-terminal portion of the molecule.
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| - | ==About this Structure==
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| - | 2DFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Micrococcus_luteus Micrococcus luteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DFW OCA].
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| - | ==Reference==
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| - | Crystal structure of a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3., Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M, Chantawannakul P, Moriguchi M, Biochem Biophys Res Commun. 2006 Aug 11;346(4):1118-24. Epub 2006 Jun 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16793004 16793004]
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| - | [[Category: Glutaminase]]
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| - | [[Category: Micrococcus luteus]]
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| - | [[Category: Single protein]]
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| - | [[Category: Chantawannakul, P.]]
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| - | [[Category: Moriguchi, M.]]
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| - | [[Category: Shirakihara, Y.]]
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| - | [[Category: Shiratori, A.]]
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| - | [[Category: Wakayama, M.]]
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| - | [[Category: Yoshimune, K.]]
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| - | [[Category: alpha/beta structure]]
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| - | [[Category: structural genomic]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:34:12 2008''
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