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| | ==Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus== | | ==Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus== |
| - | <StructureSection load='1iqp' size='340' side='right' caption='[[1iqp]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='1iqp' size='340' side='right'caption='[[1iqp]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1iqp]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IQP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IQP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1iqp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IQP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rfcs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iqp OCA], [http://pdbe.org/1iqp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1iqp RCSB], [http://www.ebi.ac.uk/pdbsum/1iqp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1iqp ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iqp OCA], [https://pdbe.org/1iqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iqp RCSB], [https://www.ebi.ac.uk/pdbsum/1iqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iqp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RFCS_PYRFU RFCS_PYRFU]] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.<ref>PMID:11274122</ref> <ref>PMID:12296822</ref> | + | [https://www.uniprot.org/uniprot/RFCS_PYRFU RFCS_PYRFU] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.<ref>PMID:11274122</ref> <ref>PMID:12296822</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 43587]] | + | [[Category: Large Structures]] |
| - | [[Category: Cann, I K.O]] | + | [[Category: Pyrococcus furiosus]] |
| - | [[Category: Ishino, S]] | + | [[Category: Cann IKO]] |
| - | [[Category: Ishino, Y]] | + | [[Category: Ishino S]] |
| - | [[Category: Morikawa, K]] | + | [[Category: Ishino Y]] |
| - | [[Category: Oyama, T]] | + | [[Category: Morikawa K]] |
| - | [[Category: Clamp loader]]
| + | [[Category: Oyama T]] |
| - | [[Category: Complex with adp]]
| + | |
| - | [[Category: Extended aaa-atpase domain]]
| + | |
| - | [[Category: Replication]]
| + | |
| Structural highlights
Function
RFCS_PYRFU Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes.
Atomic structure of the clamp loader small subunit from Pyrococcus furiosus.,Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cann IK, Ishino S, Yuasa M, Daiyasu H, Toh H, Ishino Y. Biochemical analysis of replication factor C from the hyperthermophilic archaeon Pyrococcus furiosus. J Bacteriol. 2001 Apr;183(8):2614-23. PMID:11274122 doi:http://dx.doi.org/10.1128/JB.183.8.2614-2623.2001
- ↑ Matsumiya S, Ishino S, Ishino Y, Morikawa K. Physical interaction between proliferating cell nuclear antigen and replication factor C from Pyrococcus furiosus. Genes Cells. 2002 Sep;7(9):911-22. PMID:12296822
- ↑ Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K. Atomic structure of the clamp loader small subunit from Pyrococcus furiosus. Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747
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