1k0m
From Proteopedia
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==Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms== | ==Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms== | ||
| - | <StructureSection load='1k0m' size='340' side='right' caption='[[1k0m]], [[Resolution|resolution]] 1.40Å' scene=''> | + | <StructureSection load='1k0m' size='340' side='right'caption='[[1k0m]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1k0m]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1k0m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0m OCA], [https://pdbe.org/1k0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0m RCSB], [https://www.ebi.ac.uk/pdbsum/1k0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/CLIC1_HUMAN CLIC1_HUMAN] Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.<ref>PMID:9139710</ref> <ref>PMID:10834939</ref> <ref>PMID:11195932</ref> <ref>PMID:11940526</ref> <ref>PMID:11978800</ref> <ref>PMID:11551966</ref> <ref>PMID:14613939</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0m ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0m ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | CLIC1 (NCC27) is a member of the highly conserved class of chloride ion channels that exists in both soluble and integral membrane forms. Purified CLIC1 can integrate into synthetic lipid bilayers forming a chloride channel with similar properties to those observed in vivo. The structure of the soluble form of CLIC1 has been determined at 1.4-A resolution. The protein is monomeric and structurally homologous to the glutathione S-transferase superfamily, and it has a redox-active site resembling glutaredoxin. The structure of the complex of CLIC1 with glutathione shows that glutathione occupies the redox-active site, which is adjacent to an open, elongated slot lined by basic residues. Integration of CLIC1 into the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane helix arising from residues in the vicinity of the redox-active site. The structure indicates that CLIC1 is likely to be controlled by redox-dependent processes. | ||
| - | + | ==See Also== | |
| - | + | *[[Ion channels 3D structures|Ion channels 3D structures]] | |
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== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Homo sapiens]] |
| - | [[Category: Bauskin | + | [[Category: Large Structures]] |
| - | [[Category: Breit | + | [[Category: Bauskin AR]] |
| - | [[Category: Campbell | + | [[Category: Breit SN]] |
| - | [[Category: Curmi | + | [[Category: Campbell TJ]] |
| - | [[Category: DeMaere | + | [[Category: Curmi PMG]] |
| - | [[Category: Fairlie | + | [[Category: DeMaere MZ]] |
| - | [[Category: Harrop | + | [[Category: Fairlie WD]] |
| - | [[Category: Jankova | + | [[Category: Harrop SJ]] |
| - | [[Category: Mazzanti | + | [[Category: Jankova L]] |
| - | [[Category: Pankhurst | + | [[Category: Mazzanti M]] |
| - | [[Category: Qiu | + | [[Category: Pankhurst S]] |
| - | [[Category: Reztsova | + | [[Category: Qiu MR]] |
| - | [[Category: Tonini | + | [[Category: Reztsova T]] |
| - | [[Category: Valenzuela | + | [[Category: Tonini R]] |
| - | [[Category: Warton | + | [[Category: Valenzuela SM]] |
| - | [[Category: Wu | + | [[Category: Warton K]] |
| - | + | [[Category: Wu WM]] | |
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Current revision
Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms
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Categories: Homo sapiens | Large Structures | Bauskin AR | Breit SN | Campbell TJ | Curmi PMG | DeMaere MZ | Fairlie WD | Harrop SJ | Jankova L | Mazzanti M | Pankhurst S | Qiu MR | Reztsova T | Tonini R | Valenzuela SM | Warton K | Wu WM
