|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution Structure of the Viscotoxin B== | | ==Solution Structure of the Viscotoxin B== |
| - | <StructureSection load='1jmp' size='340' side='right' caption='[[1jmp]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1jmp' size='340' side='right'caption='[[1jmp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jmp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/European_mistletoe European mistletoe]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JMP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jmp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JMP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jmp OCA], [http://pdbe.org/1jmp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jmp RCSB], [http://www.ebi.ac.uk/pdbsum/1jmp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jmp ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jmp OCA], [https://pdbe.org/1jmp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jmp RCSB], [https://www.ebi.ac.uk/pdbsum/1jmp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jmp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/THNB_VISAL THNB_VISAL]] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.[REFERENCE:3] | + | [https://www.uniprot.org/uniprot/THNB_VISAL THNB_VISAL] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.[REFERENCE:3] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 13: |
Line 14: |
| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/1jmp_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/1jmp_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
| Line 31: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: European mistletoe]] | + | [[Category: Large Structures]] |
| - | [[Category: Bernard, C]]
| + | |
| - | [[Category: Coulon, A]]
| + | |
| - | [[Category: Darbon, H]]
| + | |
| - | [[Category: Mosbah, A]]
| + | |
| - | [[Category: Rouge, P]]
| + | |
| - | [[Category: Urech, K]]
| + | |
| - | [[Category: Thionin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Viscotoxin]]
| + | |
| | [[Category: Viscum album]] | | [[Category: Viscum album]] |
| | + | [[Category: Bernard C]] |
| | + | [[Category: Coulon A]] |
| | + | [[Category: Darbon H]] |
| | + | [[Category: Mosbah A]] |
| | + | [[Category: Rouge P]] |
| | + | [[Category: Urech K]] |
| Structural highlights
Function
THNB_VISAL Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.[REFERENCE:3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Viscotoxins A2 (VA2) and B (VB) are, together with viscotoxin A3 (VA3), among the most abundant viscotoxin isoforms that occur in mistletoe-derived medicines used in anti-cancer therapy. Although these isoforms have a high degree of amino-acid-sequence similarity, they are strikingly different from each other in their in vitro cytotoxic potency towards tumour cells. First, as VA3 is the only viscotoxin whose three-dimensional (3D) structure has been solved to date, we report the NMR determination of the 3D structures of VA2 and VB. Secondly, to account for the in vitro cytotoxicity discrepancy, we carried out a comparative study of the interaction of the three viscotoxins with model membranes. Although the overall 3D structure is highly conserved among the three isoforms, some discrete structural features and associated surface properties readily account for the different affinity and perturbation of model membranes. VA3 and VA2 interact in a similar way, but the weaker hydrophobic character of VA2 is thought to be mainly responsible for the apparent different affinity towards membranes. VB is much less active than the other two viscotoxins and does not insert into model membranes. This could be related to the occurrence of a single residue (Arg25) protruding outside the hydrophobic plane formed by the two amphipathic alpha-helices, through which viscotoxins are supposed to interact with plasma membranes.
Comparative membrane interaction study of viscotoxins A3, A2 and B from mistletoe (Viscum album) and connections with their structures.,Coulon A, Mosbah A, Lopez A, Sautereau AM, Schaller G, Urech K, Rouge P, Darbon H Biochem J. 2003 Aug 15;374(Pt 1):71-8. PMID:12733989[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Coulon A, Mosbah A, Lopez A, Sautereau AM, Schaller G, Urech K, Rouge P, Darbon H. Comparative membrane interaction study of viscotoxins A3, A2 and B from mistletoe (Viscum album) and connections with their structures. Biochem J. 2003 Aug 15;374(Pt 1):71-8. PMID:12733989 doi:10.1042/BJ20030488
|
