2dla

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[[Image:2dla.gif|left|200px]]
 
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{{Structure
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==Primase large subunit amino terminal domain from Pyrococcus horikoshii==
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|PDB= 2dla |SIZE=350|CAPTION= <scene name='initialview01'>2dla</scene>, resolution 2.9&Aring;
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<StructureSection load='2dla' size='340' side='right'caption='[[2dla]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2dla]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DLA FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dla OCA], [https://pdbe.org/2dla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dla RCSB], [https://www.ebi.ac.uk/pdbsum/2dla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dla ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dla OCA], [http://www.ebi.ac.uk/pdbsum/2dla PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dla RCSB]</span>
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[https://www.uniprot.org/uniprot/PRIL_PYRHO PRIL_PYRHO] Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.[HAMAP-Rule:MF_00701]
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}}
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__TOC__
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</StructureSection>
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'''Primase large subunit amino terminal domain from Pyrococcus horikoshii'''
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[[Category: Large Structures]]
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[[Category: Pyrococcus horikoshii OT3]]
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[[Category: Ito N]]
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==Overview==
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Archaeal/eukaryotic primases form a heterodimer consisting of a small catalytic subunit (PriS) and a large subunit (PriL). The heterodimer complex synthesizes primer oligoribonucleotides that are required for chromosomal replication. Here, we describe crystallographic and biochemical studies of the N-terminal domain (NTD) of PriL (PriL(NTD); residues 1-222) that bind to PriS from a hyperthermophilic archaeon, Pyrococcus horikoshii, at 2.9 A resolution. The PriL(NTD) structure consists of two subdomains, the helix-bundle and twisted-strand domains. The latter is structurally flexible, and is expected to contain a PriS interaction site. Pull-down and surface plasmon resonance analyses of structure-based deletion and alanine scanning mutants showed that the conserved hydrophobic Tyr155-Tyr156-Ile157 region near the flexible region is the PriS-binding site, as the Y155A/Y156A/I157A mutation markedly reduces PriS binding, by 1000-fold. These findings and a structural comparison with a previously reported PriL(NTD)-PriS complex suggest that the presented alternative conformations of the twisted-strand domain facilitate the heterodimer assembly.
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==About this Structure==
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2DLA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DLA OCA].
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==Reference==
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Molecular basis for the subunit assembly of the primase from an archaeon Pyrococcus horikoshii., Ito N, Matsui I, Matsui E, FEBS J. 2007 Mar;274(5):1340-51. Epub 2007 Feb 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17286576 17286576]
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[[Category: Pyrococcus horikoshii]]
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[[Category: Single protein]]
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[[Category: Ito, N.]]
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[[Category: helix bundle]]
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[[Category: twisted beta-sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:36:09 2008''
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Current revision

Primase large subunit amino terminal domain from Pyrococcus horikoshii

PDB ID 2dla

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