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| | ==Crystal structure of isoprenoid synthase (target EFI-501974) from clostridium perfringens== | | ==Crystal structure of isoprenoid synthase (target EFI-501974) from clostridium perfringens== |
| - | <StructureSection load='3uca' size='340' side='right' caption='[[3uca]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3uca' size='340' side='right'caption='[[3uca]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uca]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UCA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uca]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UCA FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CPE1820 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uca OCA], [http://pdbe.org/3uca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uca RCSB], [http://www.ebi.ac.uk/pdbsum/3uca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uca ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uca OCA], [https://pdbe.org/3uca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uca RCSB], [https://www.ebi.ac.uk/pdbsum/3uca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uca ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8XJE0_CLOPE Q8XJE0_CLOPE] |
| - | The number of available protein sequences has increased exponentially with the advent of high-throughput genomic sequencing, creating a significant challenge for functional annotation. Here, we describe a large-scale study on assigning function to unknown members of the trans-polyprenyl transferase (E-PTS) subgroup in the isoprenoid synthase superfamily, which provides substrates for the biosynthesis of the more than 55,000 isoprenoid metabolites. Although the mechanism for determining the product chain length for these enzymes is known, there is no simple relationship between function and primary sequence, so that assigning function is challenging. We addressed this challenge through large-scale bioinformatics analysis of >5,000 putative polyprenyl transferases; experimental characterization of the chain-length specificity of 79 diverse members of this group; determination of 27 structures of 19 of these enzymes, including seven cocrystallized with substrate analogs or products; and the development and successful application of a computational approach to predict function that leverages available structural data through homology modeling and docking of possible products into the active site. The crystallographic structures and computational structural models of the enzyme-ligand complexes elucidate the structural basis of specificity. As a result of this study, the percentage of E-PTS sequences similar to functionally annotated ones (BLAST e-value </= 1e-70) increased from 40.6 to 68.8%, and the percentage of sequences similar to available crystal structures increased from 28.9 to 47.4%. The high accuracy of our blind prediction of newly characterized enzymes indicates the potential to predict function to the complete polyprenyl transferase subgroup of the isoprenoid synthase superfamily computationally.
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| - | Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.,Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD Proc Natl Acad Sci U S A. 2013 Mar 14. PMID:23493556<ref>PMID:23493556</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 3uca" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus perfringens veillon and zuber 1898]] | + | [[Category: Clostridium perfringens]] |
| - | [[Category: Almo, S C]] | + | [[Category: Large Structures]] |
| - | [[Category: Bhosle, R]] | + | [[Category: Almo SC]] |
| - | [[Category: Chowdhury, S]] | + | [[Category: Bhosle R]] |
| - | [[Category: EFI, Enzyme Function Initiative]] | + | [[Category: Chowdhury S]] |
| - | [[Category: Evans, B]] | + | [[Category: Evans B]] |
| - | [[Category: Gerlt, J A]] | + | [[Category: Gerlt JA]] |
| - | [[Category: Glenn, A Scott]]
| + | [[Category: Hammonds J]] |
| - | [[Category: Hammonds, J]] | + | [[Category: Hillerich B]] |
| - | [[Category: Hillerich, B]] | + | [[Category: Imker HJ]] |
| - | [[Category: Imker, H J]] | + | [[Category: Patskovsky Y]] |
| - | [[Category: Patskovsky, Y]] | + | [[Category: Poulter CD]] |
| - | [[Category: Poulter, C D]] | + | [[Category: Scott Glenn A]] |
| - | [[Category: Seidel, R D]] | + | [[Category: Seidel RD]] |
| - | [[Category: Toro, R]] | + | [[Category: Toro R]] |
| - | [[Category: Washington, E]] | + | [[Category: Washington E]] |
| - | [[Category: Zencheck, W D]] | + | [[Category: Zencheck WD]] |
| - | [[Category: Isoprenoid diphosphate synthase]] | + | |
| - | [[Category: Isoprenoid synthesis]]
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| - | [[Category: Structural genomic]]
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| - | [[Category: Transferase]]
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