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| | ==Crystal structure of a ChpT protein (CC_3470) from Caulobacter crescentus CB15 at 2.30 A resolution== | | ==Crystal structure of a ChpT protein (CC_3470) from Caulobacter crescentus CB15 at 2.30 A resolution== |
| - | <StructureSection load='4fmt' size='340' side='right' caption='[[4fmt]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4fmt' size='340' side='right'caption='[[4fmt]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fmt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caucr Caucr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FMT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fmt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_CB15 Caulobacter vibrioides CB15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FMT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CC_3470 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190650 CAUCR])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fmt OCA], [http://pdbe.org/4fmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fmt RCSB], [http://www.ebi.ac.uk/pdbsum/4fmt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fmt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fmt OCA], [https://pdbe.org/4fmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fmt RCSB], [https://www.ebi.ac.uk/pdbsum/4fmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fmt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9A2T6_CAUVC Q9A2T6_CAUVC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Caucr]] | + | [[Category: Caulobacter vibrioides CB15]] |
| - | [[Category: Structural genomic]] | + | [[Category: Large Structures]] |
| - | [[Category: Shapiro, L]] | + | [[Category: Shapiro L]] |
| - | [[Category: A phosphotransfer protein]]
| + | |
| - | [[Category: A two-component signaling pathway]]
| + | |
| - | [[Category: Jcsg]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q9A2T6_CAUVC
Publication Abstract from PubMed
Vital to bacterial survival is the faithful propagation of cellular signals, and in Caulobacter crescentus, ChpT is an essential mediator within the cell-cycle circuit. ChpT functions as a histidine-containing phosphotransfer protein (HPt) that shuttles a phosphoryl group from the receiver domain of CckA, the upstream hybrid histidine kinase (HK), to one of two downstream response regulators (CtrA or CpdR) that controls cell-cycle progression. To understand how ChpT interacts with multiple signaling partners, we solved the crystal structure of ChpT at 2.3 A resolution. ChpT adopts a pseudo-HK architecture but does not bind ATP. We identified two point mutation classes affecting phosphotransfer and cell morphology: one that globally impairs ChpT phosphotransfer, and a second that mediates partner selection. Importantly, a small set of conserved ChpT residues promotes signaling crosstalk and contributes to the branched signaling that activates the master regulator CtrA while inactivating the CtrA degradation signal, CpdR.
Branched signal wiring of an essential bacterial cell-cycle phosphotransfer protein.,Blair JA, Xu Q, Childers WS, Mathews II, Kern JW, Eckart M, Deacon AM, Shapiro L Structure. 2013 Sep 3;21(9):1590-601. doi: 10.1016/j.str.2013.06.024. Epub 2013, Aug 8. PMID:23932593[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Blair JA, Xu Q, Childers WS, Mathews II, Kern JW, Eckart M, Deacon AM, Shapiro L. Branched signal wiring of an essential bacterial cell-cycle phosphotransfer protein. Structure. 2013 Sep 3;21(9):1590-601. doi: 10.1016/j.str.2013.06.024. Epub 2013, Aug 8. PMID:23932593 doi:http://dx.doi.org/10.1016/j.str.2013.06.024
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