4rsm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:55, 1 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol==
==Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol==
-
<StructureSection load='4rsm' size='340' side='right' caption='[[4rsm]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+
<StructureSection load='4rsm' size='340' side='right'caption='[[4rsm]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4rsm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RSM FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4rsm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RSM FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3VB:D-THREITOL'>3VB</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSMEG_3599, MSMEI_3516 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3VB:D-THREITOL'>3VB</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsm OCA], [http://pdbe.org/4rsm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rsm RCSB], [http://www.ebi.ac.uk/pdbsum/4rsm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rsm ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsm OCA], [https://pdbe.org/4rsm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rsm RCSB], [https://www.ebi.ac.uk/pdbsum/4rsm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rsm ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/THPA_MYCS2 THPA_MYCS2] Part of an ABC transporter complex involved in D-threitol import. Binds D-threitol. Functions in the transport for the degradation pathway of D-threitol, that allows M.smegmatis to grow on this compound as the sole carbon source.<ref>PMID:26560079</ref>
-
We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol.
+
-
 
+
-
A General Strategy for the Discovery of Metabolic Pathways: d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis.,Huang H, Carter MS, Vetting MW, Al-Obaidi N, Patskovsky Y, Almo SC, Gerlt JA J Am Chem Soc. 2015 Nov 25;137(46):14570-3. doi: 10.1021/jacs.5b08968. Epub 2015 , Nov 12. PMID:26560079<ref>PMID:26560079</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4rsm" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Mycs2]]
+
[[Category: Large Structures]]
-
[[Category: Almo, S C]]
+
[[Category: Mycolicibacterium smegmatis MC2 155]]
-
[[Category: Attonito, J D]]
+
[[Category: Al Obaidi N]]
-
[[Category: Bhosle, R]]
+
[[Category: Almo SC]]
-
[[Category: Chamala, S]]
+
[[Category: Attonito JD]]
-
[[Category: Chowdhury, S]]
+
[[Category: Bhosle R]]
-
[[Category: EFI, Enzyme Function Initiative]]
+
[[Category: Chamala S]]
-
[[Category: Gerlt, J A]]
+
[[Category: Chowdhury S]]
-
[[Category: Glenn, A Scott]]
+
[[Category: Gerlt JA]]
-
[[Category: Hillerich, B]]
+
[[Category: Hillerich B]]
-
[[Category: Lafleur, J]]
+
[[Category: Lafleur J]]
-
[[Category: Love, J]]
+
[[Category: Love J]]
-
[[Category: Morisco, L L]]
+
[[Category: Morisco LL]]
-
[[Category: Obaidi, N Al]]
+
[[Category: Patskovsky Y]]
-
[[Category: Patskovsky, Y]]
+
[[Category: Scott Glenn A]]
-
[[Category: Siedel, R D]]
+
[[Category: Siedel RD]]
-
[[Category: Toro, R]]
+
[[Category: Toro R]]
-
[[Category: Wasserman, S R]]
+
[[Category: Wasserman SR]]
-
[[Category: Whalen, K L]]
+
[[Category: Whalen KL]]
-
[[Category: Abc-type]]
+
-
[[Category: Efi]]
+
-
[[Category: Enzyme function initiative]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Sugar transporter]]
+
-
[[Category: Transport protein]]
+

Current revision

Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol

PDB ID 4rsm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4rsm"
Personal tools